Publication Details |
| Category | Text Publication |
| Reference Category | Book chapters |
| Title (Primary) | A stoichiometric and kinetic model for the growth-associated microbial degradation of MTBE/ETBE |
| Title (Secondary) | MTBE and other fuel oxygenates. Proceedings of the 3rd European Conference, Antwerp, June 7-8, 2007 |
| Author | Müller, R.H.; Rohwerder, T.; Harms, H. |
| Publisher | Bastiaens, L. |
| Year | 2007 |
| Department | UMB |
| Page From | 33 |
| Page To | 37 |
| Language | englisch |
| UFZ inventory | Leipzig, Bibliothek, Hauptlesesaal, 00496940, 15-0285 |
| Abstract |
A theoretical study based on the YATP
concept was conducted to elucidate the stoichiometry of oxygenate (MTBE, ETBE) utilization
by microorganisms. Different putative pathways for aerobic substrate conversion
were taken into account. The results suggest that MTBE is an effective
heterotrophic substrate that can sustain growth yields of up to 0.87 g g-1.
However, extension of the calculation to a growth-related kinetic model revealed
the limits of productive degradation. Depending on the maintenance coefficient
m and the associated biomass decay term b,
growth-associated carbon conversion became strongly dependent from substrate
fluxes. Slow degradation kinetics predicted relatively high threshold
concentrations, Smin, below which growth would not further be
supported. Smin strongly depended on the maximum growth rate µmax
and band was directly correlated to Ks,
the half maximum rate-associated substrate concentration, meaning that any
effect impacting these parameters would also change Smin. The
interdependency of the parameters defined the conditions under which productive
degradation is possible. Limitations in defined catabolic steps in substrate
utilization might limit growth-associated substrate conversion and lead to
overflow metabolism. This should result in the excretion of metabolites or the synthesis
of storage compounds like polyhydroxybutyrates (PHB) or extracellular polymeric
substances (EPS). Synthesis of extra polymeric material (PHB, EPS) was included
in the calculation and shown to impact yield and threshold substrate
concentration in MTBE degradation. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2099 |
| Müller, R.H., Rohwerder, T., Harms, H. (2007): A stoichiometric and kinetic model for the growth-associated microbial degradation of MTBE/ETBE In: Bastiaens, L. (ed.) MTBE and other fuel oxygenates. Proceedings of the 3rd European Conference, Antwerp, June 7-8, 2007 Vito, Mol, p. 33 - 37 |
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