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Title (Primary) Anaerobic ammonium oxidation (anammox) with planktonic cells in a redox-stable semicontinuous stirred-tank reactor
Author Ding, C.; Enyi, F.O.; Adrian, L.;
Journal Environmental Science & Technology
Year 2018
Department ISOBIO;
Volume 52
Issue 10
Language englisch;
POF III (all) T41;
Supplements https://pubs.acs.org/doi/suppl/10.1021/acs.est.7b05979/suppl_file/es7b05979_si_001.pdf
Abstract Anaerobic ammonium oxidizing (anammox) bacteria are routinely cultivated in mixed culture in biomass-retaining bioreactors or as planktonic cells in membrane bioreactors. Here, we demonstrate that anammox bacteria can also be cultivated as planktonic cells in a semicontinuous stirred-tank reactor (semi-CSTR) with a specific growth rate μ of 0.33 d–1 at 30 °C. Redox potential inside the reactor stabilized at around 10 mV (±15 mV; vs standard hydrogen electrode) without gas purging. Reactor headspace pressure was used as a sensitive and real-time indicator for nitrogen evolution and anammox activity. The reactor was dominated by an organism closely related to “Candidatus Kuenenia stuttgartiensis” (∼87% abundance) as shown by Illumina amplicon sequencing and fluorescence in situ hybridization. Epifluorescence microscopy demonstrated that all cells were in their planktonic form. Mass balance analysis revealed a nitrite/ammonium ratio of 1.270, a nitrate/ammonium ratio of 0.238, and a biomass yield of 1.97 g volatile suspended solids per mole of consumed ammonium. Batch experiments with the reactor effluent showed that anammox activities were sensitive to sulfide (IC50 = 5 μM) and chloramphenicol (IC50 = 19 mg L–1), much lower than reported for granular anammox biomass. This study shows that semi-CSTR is a powerful tool to study anammox bacteria.
ID 20400
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20400
Ding, C., Enyi, F.O., Adrian, L. (2018):
Anaerobic ammonium oxidation (anammox) with planktonic cells in a redox-stable semicontinuous stirred-tank reactor
Environ. Sci. Technol. 52 (10), 5671 - 5681