Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.jprot.2018.03.030 |
| Document | author version |
| Title (Primary) | A retentive memory of tetrachloroethene respiration in Sulfurospirillum halorespirans - involved proteins and a possible link to acetylation of a two-component regulatory system |
| Author | Türkowsky, D.; Esken, J.; Goris, T.; Schubert, T.; Diekert, G.; Jehmlich, N.
; von Bergen, M. |
| Source Titel | Journal of Proteomics |
| Year | 2018 |
| Department | MOLSYB |
| Volume | 181 |
| Page From | 36 |
| Page To | 46 |
| Language | englisch |
| Keywords | Regulation; Two-component regulatory system; Acetylomics; Proteomics; Bioremediation; Dehalorespiration |
| Abstract | Organohalide respiration (OHR), comprising the reductive dehalogenation of
halogenated organic compounds, is subject to a unique memory effect and
long-term transcriptional downregulation of the involved genes in Sulfurospirillum
multivorans. Gene expression ceases slowly over approximately 100
generations in the absence of tetrachloroethene (PCE). However, the molecular
mechanisms of this regulation process are not understood. We show here that Sulfurospirillum
halorespirans undergoes the same type of regulation when cultivated
without chlorinated ethenes for a long period of time. In addition, we compared
the proteomes of S. halorespirans cells cultivated in the presence of
PCE with those of cells long- and short-term cultivated with nitrate as the
sole electron acceptor. Important OHR-related proteins previously unidentified
in S. multivorans include a histidine kinase, a putative quinol dehydrogenase
membrane protein, and a PCE-induced porin. Since for some regulatory proteins a
posttranslational regulation of activity by lysine acetylations is known, we
also analyzed the acetylome of S. halorespirans, revealing that 32% of
the proteome was acetylated in at least one condition. The data indicate that
the response regulator and the histidine kinase of a two-component system most
probably involved in induction of PCE respiration are highly acetylated during
short-term cultivation with nitrate in the absence of PCE.
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| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20394 |
| Türkowsky, D., Esken, J., Goris, T., Schubert, T., Diekert, G., Jehmlich, N., von Bergen, M. (2018): A retentive memory of tetrachloroethene respiration in Sulfurospirillum halorespirans - involved proteins and a possible link to acetylation of a two-component regulatory system J. Proteomics 181 , 36 - 46 10.1016/j.jprot.2018.03.030 |
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