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Reference Category Book chapters
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Title (Primary) Cis-trans isomerase of unsaturated fatty acids: an immediate bacterial adaptive mechanism to cope with emerging membrane perturbation caused by toxic hydrocarbons
Title (Secondary) Cellular ecophysiology of microbe: Hydrocarbon and lipid interactions
Author Heipieper, H.J.; Fischer, J.; Meinhardt, F.;
Publisher Krell, T.;
Journal Handbook of Hydrocarbon and Lipid Microbiology
Year 2018
Department UBT;
Language englisch;
POF III (all) T41;
Abstract

A rather efficient solvent adaptation mechanism enabling several gram-negative bacteria to tolerate and grow in the presence of membrane-disturbing compounds is the isomerization of cis- to trans-unsaturated membrane fatty acids. The degree of isomerization obviously depends on the toxicity and the concentration of membrane-affecting agents. Synthesis of trans-fatty acids comes about by direct isomerization of the respective cis-configuration of the double bond without shifting the position. The purpose of the conversion of the cis-configuration to trans is apparently the rapid adaptation of the membrane fluidity to rising temperature or the presence of toxic organic hydrocarbons.

The cis–trans-isomerase (Cti) is a constitutively expressed periplasmic enzyme that – to exert its action – necessitates neither ATP nor other cofactors, and consistently, is independent of de novo synthesis of lipids. A heme-binding site typical of cytochrome c-type proteins is present in the predicted Cti polypeptide indicating a reaction mechanism that renounces temporary saturation of the double bond. Due to its direct correlation with toxicity, cis–trans-isomerization is a potential biomarker for recording solvent stress or changes of other environmental conditions.

ID 20372
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20372
Heipieper, H.J., Fischer, J., Meinhardt, F. (2018):
Cis-trans isomerase of unsaturated fatty acids: an immediate bacterial adaptive mechanism to cope with emerging membrane perturbation caused by toxic hydrocarbons
In: Krell, T. (ed.)
Cellular ecophysiology of microbe: Hydrocarbon and lipid interactions
Handbook of Hydrocarbon and Lipid Microbiology
Springer International Publishing, Cham, p. 385 - 395