Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1038/s41598-018-22617-x |
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| Title (Primary) | A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways |
| Author | Atashgahi, S.; Hornung, B.; van der Waals, M.J.; Nunes da Rocha, U.; Hugenholtz, F.; Nijsse, B.; Molenaar, D.; van Spanning, R.; Stams, A.J.M.; Gerritse, J.; Smidt, H. |
| Source Titel | Scientific Reports |
| Year | 2018 |
| Department | UMB |
| Volume | 8 |
| Page From | art. 4490 |
| Language | englisch |
| Supplements | Supplement 1 Supplement 2 |
| UFZ wide themes | RU3; |
| Abstract | In this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family Peptococcaceae dominated all samples (21–36% relative abundance) indicating their key role in the community. We found a highly transcribed gene cluster encoding a presumed anaerobic benzene carboxylase (AbcA and AbcD) and a benzoate-coenzyme A ligase (BzlA). Predicted gene products showed >96% amino acid identity and similar gene order to the corresponding benzene degradation gene cluster described previously, providing further evidence for anaerobic benzene activation via carboxylation. For subsequent benzoyl-CoA dearomatization, bam-like genes analogous to the ones found in other strict anaerobes were transcribed, whereas gene transcripts involved in downstream benzoyl-CoA degradation were mostly analogous to the ones described in facultative anaerobes. The concurrent transcription of genes encoding enzymes involved in oxygenase-mediated aerobic benzene degradation suggested oxygen presence in the culture, possibly formed via a recently identified nitric oxide dismutase (Nod). Although we were unable to detect transcription of Nod-encoding genes, addition of nitrite and formate to the continuous culture showed indication for oxygen production. Such an oxygen production would enable aerobic microbes to thrive in oxygen-depleted and nitrate-containing subsurface environments contaminated with hydrocarbons. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20142 |
| Atashgahi, S., Hornung, B., van der Waals, M.J., Nunes da Rocha, U., Hugenholtz, F., Nijsse, B., Molenaar, D., van Spanning, R., Stams, A.J.M., Gerritse, J., Smidt, H. (2018): A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways Sci. Rep. 8 , art. 4490 10.1038/s41598-018-22617-x |
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