Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s41598-018-21039-z
Licence creative commons licence
Title (Primary) Identification of T helper (Th)1- and Th2-associated antigens of Cryptococcus neoformans in a murine model of pulmonary infection
Author Firacative, C.; Gressler, A.E.; Schubert, K.; Schulze, B.; Müller, U.; Brombacher, F.; von Bergen, M.; Alber, G.
Source Titel Scientific Reports
Year 2018
Department MOLSYB
Volume 8
Page From art. 2681
Language englisch
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-018-21039-z/MediaObjects/41598_2018_21039_MOESM1_ESM.pdf
Keywords Infection; Lymphocyte differentiation
UFZ wide themes RU3;
Abstract Cryptococcosis, caused by Cryptococcus neoformans, has been demonstrated to be controlled by T helper (Th)1 cells while Th2 cells are associated with fungal growth and dissemination. Although cryptococcal immunoreactive protein antigens were previously identified, their association with Th1 or Th2 immune responses was not provided. In mice, Th1-dependent IFN-γ induces the production of IgG2a, whereas the Th2 cytokine IL-4 stimulates the expression of IgG1 rendering each isotype an indicator of the underlying Th cell response. Therefore, we performed an immunoproteomic study that distinguishes Th1- and Th2-associated antigens by their reactivity with Th1-dependent IgG2a or Th2-dependent IgG1 antibodies in sera from C. neoformans-infected wild-type mice. We additionally analysed sera from Th2-prone IL-12-deficient and Th1-prone IL-4Rα-deficient mice extending the results found in wild-type mice. In total, ten, four, and three protein antigens associated with IgG1, IgG2a, or both isotypes, respectively, were identified. Th2-associated antigens represent promising candidates for development of immunotherapy regimens, whereas Th1-associated antigens may serve as candidates for vaccine development. In conclusion, this study points to intrinsic immunomodulatory effects of fungal antigens on the process of Th cell differentiation based on the identification of cryptococcal protein antigens specifically associated with Th1 or Th2 responses throughout mice of different genotypes.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19951
Firacative, C., Gressler, A.E., Schubert, K., Schulze, B., Müller, U., Brombacher, F., von Bergen, M., Alber, G. (2018):
Identification of T helper (Th)1- and Th2-associated antigens of Cryptococcus neoformans in a murine model of pulmonary infection
Sci. Rep. 8 , art. 2681 10.1038/s41598-018-21039-z