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Title (Primary) Comparison of lung proteome profiles in two rodent models of pulmonary arterial hypertension
Author Laudi, S.; Steudel, W.; Jonscher, K.; Schöning, W.; Schniedewind, B.; Kaisers, U.; Christians, U.; Trump, S.;
Journal Proteomics
Year 2007
Department IMMU;
Volume 7
Issue 14
Language englisch;
Keywords Hypoxia; Monocrotaline; Protein expression analysis; Pulmonary arterial hypertension
Abstract We studied the lung proteome changes in two widely used models of pulmonary arterial hypertension (PAH): monocrotaline (MCT) injection and chronic hypoxia (CH); untreated rats were used as controls (n = 6/group). After 28 days, invasive right ventricular systolic pressure (RVSP) was measured. Lungs were immunostained for -smooth muscle actin (SMA). 2-DE (n = 4/group) followed by nano-LC-MS/MS was applied for protein identification. Western blotting was used additionally if possible. RVSP was significantly increased in MCT- and CH-rats (MCT 62.5 ± 4.4 mmHg, CH 62.2 ± 4.1 mmHg, control 25.0 ± 1.7 mmHg, p<0.001). This was associated with an increase of SMA positive vessels. In both groups, there was a significantly increased expression of proteins associated with the contractile apparatus (diphosphoHsp27 (p<0.001), Septin2 (p<0.001), F-actin capping protein (p<0.01), and tropomyosin (p<0.02)). In CH, proteins of the nitric oxide (Hsc70; p = 0.002), carbon monoxide (biliverdin reductase; p = 0.005), and vascular endothelial growth factor (VEGF) pathway (annexin 3; p<0.001) were significantly increased. In MCT, proteins involved in serotonin synthesis (14-3-3; p = 0.02), the enhanced unfolded protein response (ERp57; p = 0.02), and intracellular chloride channels (CLIC 1; p = 0.002) were significantly elevated. Therefore, MCT- and CH-induced vasoconstriction and remodeling seemed to be mediated via different signaling pathways. These differences should be considered in future studies using either PAH model.
ID 1991
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1991
Laudi, S., Steudel, W., Jonscher, K., Schöning, W., Schniedewind, B., Kaisers, U., Christians, U., Trump, S. (2007):
Comparison of lung proteome profiles in two rodent models of pulmonary arterial hypertension
Proteomics 7 (14), 2469 - 2478