Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00253-017-8706-6 |
Licence  |
|
| Title (Primary) | Formulation and stabilization of an Arthrobacter strain with good storage stability and 4-chlorophenol-degradation activity for bioremediation |
| Author | Bjerketorp, J.; Röling, W.F.M.; Feng, X.-M.; Hernández Garcia, A.; Heipieper, H.J.
; Håkansson, S. |
| Source Titel | Applied Microbiology and Biotechnology |
| Year | 2018 |
| Department | UBT |
| Volume | 102 |
| Issue | 4 |
| Page From | 2031 |
| Page To | 2040 |
| Language | englisch |
| Keywords | Bioremediation; Bioaugmentation; Arthrobacter; Formulation; Microbial stabilization |
| UFZ wide themes | RU3; |
| Abstract | Chlorophenols are widespread and of environmental concern due to their toxic and carcinogenic properties. Development of less costly and less technically challenging remediation methods are needed; therefore, we developed a formulation based on micronized vermiculite that, when air-dried, resulted in a granular product containing the 4-chlorophenol (4-CP)-degrading Gram-positive bacterium Arthrobacter chlorophenolicus A6. This formulation and stabilization method yielded survival rates of about 60% that remained stable in storage for at least 3 months at 4 °C. The 4-CP degradation by the formulated and desiccated A. chlorophenolicus A6 cells was compared to that of freshly grown cells in controlled-environment soil microcosms. The stabilized cells degraded 4-CP equally efficient as freshly grown cells in two different set-ups using both hygienized and non-treated soils. The desiccated microbial product was successfully employed in an outdoor pot trial showing its effectiveness under more realistic environmental conditions. No significant phytoremediation effects on 4-CP degradation were observed in the outdoor pot experiment. The 4-CP degradation kinetics from both the microcosms and the outdoor pot trial were used to generate a predictive model of 4-CP biodegradation potentially useful for larger-scale operations, enabling better bioremediation set-ups and saving of resources. This study also opens up the possibility of formulating and stabilizing also other Arthrobacter strains possessing different desirable pollutant-degrading capabilities. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19862 |
| Bjerketorp, J., Röling, W.F.M., Feng, X.-M., Hernández Garcia, A., Heipieper, H.J., Håkansson, S. (2018): Formulation and stabilization of an Arthrobacter strain with good storage stability and 4-chlorophenol-degradation activity for bioremediation Appl. Microbiol. Biotechnol. 102 (4), 2031 - 2040 10.1007/s00253-017-8706-6 |
|