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Title (Primary) A patchwork pathway for oxygenase-independent degradation of side chain containing steroids
Author Warnke, M.; Jacoby, C.; Jung, T.; Agne, M.; Mergelsberg, M.; Starke, R.; Jehmlich, N.; von Bergen, M.; Richnow, H.-H.; Brüls, T.; Boll, M.;
Journal Environmental Microbiology
Year 2017
Department ISOBIO; MOLSYB;
Volume 19
Issue 11
Language englisch;
POF III (all) T41;
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UFZ wide themes RU3;
Abstract The denitrifying betaproteobacterium Sterolibacterium denitrificans serves as model organism for studying the oxygen-independent degradation of cholesterol. Here, we demonstrate its capability of degrading various globally abundant side chain containing zoo-, phyto- and mycosterols. We provide the complete genome that empowered an integrated genomics/proteomics/metabolomics approach, accompanied by the characterization of a characteristic enzyme of steroid side chain degradation. The results indicate that individual molybdopterin-containing steroid dehydrogenases are involved in C25-hydroxylations of steroids with different isoprenoid side chains, followed by the unusual conversion to C26-oic acids. Side chain degradation to androsta-1,4-diene-3,17-dione (ADD) via aldolytic C–C bond cleavages involves acyl-CoA synthetases/dehydrogenases specific for the respective 26-, 24- and 22-oic acids/-oyl-CoAs and promiscuous MaoC-like enoyl-CoA hydratases, aldolases and aldehyde dehydrogenases. Degradation of rings A and B depends on gene products uniquely found in anaerobic steroid degraders, which after hydrolytic cleavage of ring A, again involves CoA-ester intermediates. The degradation of the remaining CD rings via hydrolytic cleavage appears to be highly similar in aerobic and anaerobic bacteria. Anaerobic cholesterol degradation employs a composite repertoire of more than 40 genes partially known from aerobic degradation in gammaproteobacteria/actinobacteria, supplemented by unique genes that are required to circumvent oxygenase-dependent reactions.
ID 19784
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19784
Warnke, M., Jacoby, C., Jung, T., Agne, M., Mergelsberg, M., Starke, R., Jehmlich, N., von Bergen, M., Richnow, H.-H., Brüls, T., Boll, M. (2017):
A patchwork pathway for oxygenase-independent degradation of side chain containing steroids
Environ. Microbiol. 19 (11), 4684 - 4699