Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1080/20002297.2017.1379826
Title (Primary) Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow
Author Frost-Kristensen, M.F.; Zeng, G.; Neu, T.R.; Meyer, R.L.; Baelum, V.; Schlafer, S.
Journal Journal of Oral Microbiology
Year 2017
Department FLOEK
Volume 9
Issue 1
Page From art. 1379826
Language englisch
Keywords Adhesion; atomic force microscopy; biofilms; caseinoglycomacropeptide; dental caries; fluorescence lectin-barcoding; fluorescence lectin-binding analysis; milk protein; osteopontin; single-cell force spectroscopy
UFZ wide themes RU2;
Abstract The bovine milk protein osteopontin (OPN) may be an efficient means to prevent bacterial adhesion to dental tissues and control biofilm formation. This study sought to determine to what extent OPN impacts adhesion forces and surface attachment of different bacterial strains involved in dental caries or medical device–related infections. It further investigated if OPN’s effect on adhesion is caused by blocking the accessibility of glycoconjugates on bacterial surfaces. Bacterial adhesion was determined in a shear-controlled flow cell system in the presence of different concentrations of OPN, and interaction forces of single bacteria were quantified using single-cell force spectroscopy before and after OPN exposure. Moreover, the study investigated OPN’s effect on the accessibility of cell surface glycoconjugates through fluorescence lectin-binding analysis. OPN strongly affected bacterial adhesion in a dose-dependent manner for all investigated species (Actinomyces naeslundii, Actinomyces viscosus, Lactobacillus paracasei subsp. paracasei, Staphylococcus epidermidis, Streptococcus mitis, and Streptococcus oralis). Likewise, adhesion forces decreased after OPN treatment. No effect of OPN on the lectin-accessibility to glycoconjugates was found. OPN reduces the adhesion and adhesion force/energy of a variety of bacteria and has a potential therapeutic use for biofilm control. OPN acts upon bacterial adhesion without blocking cell surface glycoconjugates.
Persistent UFZ Identifier
Frost-Kristensen, M.F., Zeng, G., Neu, T.R., Meyer, R.L., Baelum, V., Schlafer, S. (2017):
Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow
J. Oral Microbiol. 9 (1), art. 1379826