Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1128/mSystems.00038-17 |
| Title (Primary) | Vitamin and amino acid auxotrophy in anaerobic consortia operating under methanogenic conditions |
| Author | Hubalek, V.; Buck, M.; Tan, B.; Foght, J.; Wendeberg, A.; Berry, D.; Bertilsson, S.; Eiler, A. |
| Journal | mSystems |
| Year | 2017 |
| Department | UMB |
| Volume | 2 |
| Issue | 5 |
| Page From | e00038-17 |
| Language | englisch |
| Supplements | https://msystems.asm.org/content/msys/2/5/e00038-17/DC1/embed/inline-supplementary-material-1.pdf?download=true https://msystems.asm.org/content/msys/2/5/e00038-17/DC2/embed/inline-supplementary-material-2.pdf?download=true https://msystems.asm.org/content/msys/2/5/e00038-17/DC3/embed/inline-supplementary-material-3.pdf?download=true https://msystems.asm.org/content/msys/2/5/e00038-17/DC4/embed/inline-supplementary-material-4.pdf?download=true https://msystems.asm.org/content/msys/2/5/e00038-17/DC5/embed/inline-supplementary-material-5.pdf?download=true https://msystems.asm.org/content/msys/2/5/e00038-17/DC6/embed/inline-supplementary-material-6.pdf?download=true |
| Keywords | Black Queen hypothesis; metagenomics; petroleum; syntrophy |
| UFZ wide themes | RU3; |
| Abstract | Syntrophy among Archaea and Bacteria facilitates the anaerobic degradation of organic compounds to CH4 and CO2.
Particularly during aliphatic and aromatic hydrocarbon mineralization,
as in the case of crude oil reservoirs and petroleum-contaminated
sediments, metabolic interactions between obligate mutualistic microbial
partners are of central importance. Using micromanipulation combined
with shotgun metagenomic approaches, we describe the genomes of complex
consortia within short-chain alkane-degrading cultures operating under
methanogenic conditions. Metabolic reconstruction revealed that only a
small fraction of genes in the metagenome-assembled genomes encode the
capacity for fermentation of alkanes facilitated by energy conservation
linked to H2 metabolism. Instead, the presence of inferred
lifestyles based on scavenging anabolic products and intermediate
fermentation products derived from detrital biomass was a common
feature. Additionally, inferred auxotrophy for vitamins and amino acids
suggests that the hydrocarbon-degrading microbial assemblages are
structured and maintained by multiple interactions beyond the canonical H2-producing
and syntrophic alkane degrader-methanogen partnership. Compared to
previous work, our report points to a higher order of complexity in
microbial consortia engaged in anaerobic hydrocarbon transformation. IMPORTANCE Microbial interactions between Archaea and Bacteria mediate many important chemical transformations in the biosphere from degrading abundant polymers to synthesis of toxic compounds. Two of the most pressing issues in microbial interactions are how consortia are established and how we can modulate these microbial communities to express desirable functions. Here, we propose that public goods (i.e., metabolites of high energy demand in biosynthesis) facilitate energy conservation for life under energy-limited conditions and determine the assembly and function of the consortia. Our report suggests that an understanding of public good dynamics could result in new ways to improve microbial pollutant degradation in anaerobic systems. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19563 |
| Hubalek, V., Buck, M., Tan, B., Foght, J., Wendeberg, A., Berry, D., Bertilsson, S., Eiler, A. (2017): Vitamin and amino acid auxotrophy in anaerobic consortia operating under methanogenic conditions mSystems 2 (5), e00038-17 |
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