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Reference Category Journals
DOI 10.1111/exd.13374
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Title (Primary) Syndecan-1 regulates dendritic cell migration in cutaneous hypersensitivity to haptens
Author Averbeck, M.; Kuhn, S.; Bühligen, J.; Götte, M.; Simon, J.C.; Polte, T.
Source Titel Experimental Dermatology
Year 2017
Department IMMU
Volume 26
Issue 11
Page From 1060
Page To 1067
Language englisch
Keywords cell migration; cutaneous hypersensitivity; dendritic cell; syndecan
UFZ wide themes RU3;
Abstract In human dendritic cells (DCs), we previously demonstrated in vitro that syndecan-1 (SDC1) is downregulated during maturation correlating with enhanced motility. We investigated the effects of SDC1 on DC migration in vivo during TNCB(2,4,6-trinitro-1-chlorobenzene)-induced cutaneous hypersensitivity reaction (CHS) in mice. We show that DC in SDC1-deficient mice migrated faster and at a higher rate to lymph nodes draining the hapten-painted skin. Adoptive transfer of SDC1-deficient hapten- and fluorochrome-labelled DC into wild-type (WT) mice led to increased and faster migration of DC to paracortical lymph nodes, and to a stronger CHS compared to WT DC. In SDC1-/- mice, CCR7 remains longer on the DC surface within the first 15-minutes maturation (after LPS-induced maturation). In addition, a time-dependent upregulation of CCL2, CCL3, VCAM1 and talin was found during maturation in SDC1-/- DC. However, no difference in T-cell-stimulating capacity of SDC1-deficient DC was found compared to WT DC. Mechanistically, SDC1-deficient DC showed enhanced migration towards CCL21 and CCL19. This may result from functional overexpression of CCR7 in SDC1-/- DC. Increased and accelerated migration of otherwise functionally intact SDC1-deficient DC leads to an exacerbated CHS. Based on our results, we conclude that SDC1 on DC negatively regulates DC migration.
Persistent UFZ Identifier
Averbeck, M., Kuhn, S., Bühligen, J., Götte, M., Simon, J.C., Polte, T. (2017):
Syndecan-1 regulates dendritic cell migration in cutaneous hypersensitivity to haptens
Exp. Dermatol. 26 (11), 1060 - 1067 10.1111/exd.13374