Publication Details

Reference Category Journals
DOI / URL link
Document Shareable Link
Title (Primary) Phthaloyl-coenzyme A decarboxylase from Thauera chlorobenzoica: the prenylated flavin-, K+- and Fe2+-dependent key enzyme of anaerobic phthalate degradation
Author Mergelsberg, M.; Willistein, M.; Meyer, H.; Stärk, H.-J.; Bechtel, D.F.; Pierik, A.J.; Boll, M.
Journal Environmental Microbiology
Year 2017
Department ANA
Volume 19
Issue 9
Page From 3734
Page To 3744
Language englisch
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.13875&attachmentId=183567801
UFZ wide themes RU4;
Abstract The degradation of the industrially produced and environmentally relevant phthalate esters by microorganisms is initiated by the hydrolysis to alcohols and phthalate (1,2-dicarboxybenzene). In the absence of oxygen the further degradation of phthalate proceeds via activation to phthaloyl-CoA followed by decarboxylation to benzoyl-CoA. Here, we report on the first purification and characterization of a phthaloyl-CoA decarboxylase (PCD) from the denitrifying Thauera chlorobenzoica. Hexameric PCD belongs to the UbiD-family of (de)carboxylases and contains prenylated FMN (prFMN), K+ and, unlike other UbiD-like enzymes, Fe2+ as cofactors. The latter is suggested to be involved in oxygen-independent electron-transfer during oxidative prFMN maturation. Either oxidation to the Fe3+-state in air or removal of K+ by desalting resulted in >92% loss of both, prFMN and decarboxylation activity suggesting the presence of an active site prFMN/Fe2+/K+-complex in PCD. The PCD-catalysed reaction was essentially irreversible: neither carboxylation of benzoyl-CoA in the presence of 2 M bicarbonate, nor an isotope exchange of phthaloyl-CoA with 13C-bicarbonate was observed. PCD differs in many aspects from prFMN-containing UbiD-like decarboxylases and serves as a biochemically accessible model for the large number of UbiD-like (de)carboxylases that play key roles in the anaerobic degradation of environmentally relevant aromatic pollutants.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19285
Mergelsberg, M., Willistein, M., Meyer, H., Stärk, H.-J., Bechtel, D.F., Pierik, A.J., Boll, M. (2017):
Phthaloyl-coenzyme A decarboxylase from Thauera chlorobenzoica: the prenylated flavin-, K+- and Fe2+-dependent key enzyme of anaerobic phthalate degradation
Environ. Microbiol. 19 (9), 3734 - 3744