Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1111/j.1574-6941.2007.00322.x
Title (Primary) Diversity of laccase-like multicopper oxidase genes in Morchellaceae: identification of genes potentially involved in extracellular activities related to plant litter decay
Author Kellner, H.; Luis, P.; Buscot, F.
Source Titel FEMS Microbiology Ecology
Year 2007
Department BOOEK
Volume 61
Issue 1
Page From 153
Page To 163
Language englisch
Keywords aromatic compound degradation; expression; gene diversity; laccase-like multicopper oxidase; Morchella
Abstract Despite the important role played by soil-inhabiting ascomycetes in plant litter decay processes, studies on the diversity and function of their laccase-like multicopper oxidase (LMCO) genes are scarce. In the present work, the LMCO gene diversity in 15 strains representing nine Morchellaceae and one Discinaceae species was evaluated by PCR. One to six different genes were found within the species, representing 26 different sequence types. Cluster analysis revealed LMCO genes belonging to four main gene families encoding different protein classes (Class I-IV). To identify the genes related to extracellular activities and potentially involved in litter decay processes, liquid cultures were induced by different aromatic compounds. Morchella conica and Verpa conica showed the strongest LMCO activity enhancement in the presence of the naturally occurring phenolic compound guaiacol, and their expressed LMCO genes were identified by sequencing. Only genes belonging to the gene families encoding the Class II and III proteins were expressed. Both genes (Class II and III) of the mycorrhizal-like strain M. conica were exclusively expressed in the presence of guaiacol. In contrast to the saprotrophic strain V. conica, the gene encoding the Class III protein was constitutively expressed as it was also found in control cultures without guaiacol.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1924
Kellner, H., Luis, P., Buscot, F. (2007):
Diversity of laccase-like multicopper oxidase genes in Morchellaceae: identification of genes potentially involved in extracellular activities related to plant litter decay
FEMS Microbiol. Ecol. 61 (1), 153 - 163 10.1111/j.1574-6941.2007.00322.x