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Title (Primary) Improved performance of Pseudomonas putida in a bioelectrochemical system through overexpression of periplasmic glucose dehydrogenase
Author Yu, S.; Lai, B.; Plan, M.R.; Hodson, M.P.; Lestari, E.A.; Song, H.; Krömer, J.O.;
Journal Biotechnology and Bioengineering
Year 2018
Department SOMA;
Volume 115
Issue 1
Language englisch;
POF III (all) R61;
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fbit.26433&attachmentId=196678678
Keywords Bioelectrochemical system; anode respiration; Microbial electrosynthesis; membrane-bound dehydrogenase; Pseudomonas putida
UFZ wide themes RU4;
Abstract It was recently demonstrated that a bioelectrochemical system (BES) with a redox mediator allowed Pseudomonas putida to perform anoxic metabolism, converting sugar to sugar acids with high yield. However, the low productivity currently limits the application of this technology. To improve productivity the strain was optimized through improved expression of glucose dehydrogenase (GCD) and gluconate dehydrogenase (GAD). In addition, quantitative real-time RT-PCR analysis revealed the intrinsic self-regulation of GCD and GAD. Utilizing this self-regulation system, the single overexpression strain (GCD) gave an outstanding performance in the electron transfer rate and 2-ketogluconic acid (2KGA) productivity. The peak anodic current density, specific glucose uptake rate and 2KGA producing rate were 0.12 mA/cm2, 0.27 ± 0.02 mmol/gCDW/h and 0.25 ± 0.02 mmol/gCDW/h, which were 327%, 477% and 644% of the values of wild type P. putida KT2440, respectively. This work demonstrates that expression of periplasmic dehydrogenases involved in electron transfer can significantly improve productivity in the BES.
ID 19223
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19223
Yu, S., Lai, B., Plan, M.R., Hodson, M.P., Lestari, E.A., Song, H., Krömer, J.O. (2018):
Improved performance of Pseudomonas putida in a bioelectrochemical system through overexpression of periplasmic glucose dehydrogenase
Biotechnol. Bioeng. 115 (1), 145 - 155