Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.watres.2017.05.068 |
| Title (Primary) | The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp. |
| Author | Pronk, M.; Neu, T.R.; van Loosdrecht, M.C.M.; Lin, Y.M. |
| Journal | Water Research |
| Year | 2017 |
| Department | FLOEK |
| Volume | 122 |
| Page From | 148 |
| Page To | 158 |
| Language | englisch |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S0043135417304323-mmc1.docx |
| Keywords | Defluviicoccus Aerobic granular sludge Extracellular polymeric substances Biofilm EPS extraction |
| UFZ wide themes | RU2; |
| Abstract | A new acid soluble extracellular polymeric substance (acid soluble EPS)
was extracted from an acetate fed aerobic granular sludge reactor
operated at 35 °C. Acid soluble EPS dominated granules exhibited a
remarkable and distinctive tangled tubular morphology. These granules
are dominated by Defluviicoccus Cluster II organisms. Acetic
acid instead of the usually required alkaline extraction medium was
needed to dissolve the granules and solubilise the polymeric matrix. The
extracted acid soluble EPS was analysed and identified using various
instrumental analysis including 1H and 13C Nuclear
Magnetic Resonance, Fourier Transform Infrared Spectroscopy and Raman
spectroscopy. In addition, the glycoconjugates were characterized by
fluorescence lectin-binding analysis. The acid soluble EPS is α-(1 → 4)
linked polysaccharide, containing both glucose and galactose as
monomers. There are  OCH3
groups connected to the glucose monomer. Transmission and scanning
electron microscopy (TEM, SEM) as well as confocal laser scanning
microscopy (CLSM) showed that the acid soluble EPS was present as a
tightly bound capsular EPS around bacterial cells ordered into a
sarcinae-like growth pattern. The special granule morphology is decided
by the acid soluble EPS produced by Defluviicoccus Cluster II
organisms. This work shows that no single one method can be used to
extract all possible extracellular polymeric substances. Results
obtained here can support the elucidation of biofilm formation and
structure in future research. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19160 |
| Pronk, M., Neu, T.R., van Loosdrecht, M.C.M., Lin, Y.M. (2017): The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp. Water Res. 122 , 148 - 158 |
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