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Title (Primary) Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae
Author Averesch, N.J.H.; Prima, A.; Krömer, J.O.;
Journal Bioprocess and Biosystems Engineering
Year 2017
Department SOMA;
Volume 40
Issue 8
Language englisch;
POF III (all) R61;
Keywords Para-hydroxybenzoic acid; Shikimate pathway; Saccharomyces cerevisiae; Fed-batch
UFZ wide themes RU4;
Abstract Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized. Addressing both we show that S. cerevisiae is well-suited for over-production of aromatic compounds, which are valuable in chemical industry and are particularly useful in space technology. Specifically, a strain engineered to accumulate chorismate was optimized for formation of para-hydroxybenzoic acid. Then a fed-batch bioreactor process was developed, which delivered a final titer of 2.9 g/L, a maximum rate of 18.625 mgpHBA/(gCDW × h) and carbon-yields of up to 3.1 mgpHBA/gglucose.
ID 19009
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19009
Averesch, N.J.H., Prima, A., Krömer, J.O. (2017):
Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae
Bioprocess. Biosyst. Eng. 40 (8), 1283 - 1289