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Title (Primary) Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
Author Menger, J.; Gerth, M.; Unrein, J.; Henle, K.; Schlegel, M.;
Journal Wilson Journal of Ornithology
Year 2017
Department NSF; iDiv;
Volume 129
Issue 2
Language englisch;
POF III (all) T12;
Keywords Amazonia, ant-following birds, cross-amplification, next-generation sequencing, nuclear markers
UFZ wide themes RU1
Abstract Fourteen microsatellite loci were characterized for the Rufous-throated Antbird (Thamnophilidae: Gymnopithys rufigula), an Amazonian obligate ant-following bird. Eight novel polymorphic microsatellite loci were isolated using next-generation sequencing and six additional loci were cross-amplified using primers previously designed for other bird species. All 14 loci were screened using 40 samples from central Amazon. The number of alleles per locus ranged from 3 to 20, whilst the observed and expected heterozygosities varied from 0.200 to 0.975, and 0.186 to 0.928, respectively. No linkage disequilibrium was found among the loci. Only one locus deviated from Hardy-Weinberg equilibrium. Among the eight novel loci, six were cross-amplified in at least one additional Thamnophilidae species, whilst three loci cross-amplified in two other Dendrocolaptidae species. This set of markers will be useful for future population genetic structure and parentage analysis of Amazonian forest birds.
ID 18994
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18994
Menger, J., Gerth, M., Unrein, J., Henle, K., Schlegel, M. (2017):
Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
Wilson J. Ornithol. 129 (2), 407 - 411