Publication Details
Category Text Publication
Reference Category Journals
DOI 10.1676/16-062.1
Title (Primary) Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
Author Menger, J.; Gerth, M.; Unrein, J.; Henle, K.; Schlegel, M.
Source Titel Wilson Journal of Ornithology
Year 2017
Department NSF; iDiv
Volume 129
Issue 2
Page From 407
Page To 411
Language englisch
Keywords Amazonia, ant-following birds, cross-amplification, next-generation sequencing, nuclear markers
UFZ wide themes RU1
Abstract Fourteen microsatellite loci were characterized for the Rufous-throated Antbird (Thamnophilidae: Gymnopithys rufigula), an Amazonian obligate ant-following bird. Eight novel polymorphic microsatellite loci were isolated using next-generation sequencing and six additional loci were cross-amplified using primers previously designed for other bird species. All 14 loci were screened using 40 samples from central Amazon. The number of alleles per locus ranged from 3 to 20, whilst the observed and expected heterozygosities varied from 0.200 to 0.975, and 0.186 to 0.928, respectively. No linkage disequilibrium was found among the loci. Only one locus deviated from Hardy-Weinberg equilibrium. Among the eight novel loci, six were cross-amplified in at least one additional Thamnophilidae species, whilst three loci cross-amplified in two other Dendrocolaptidae species. This set of markers will be useful for future population genetic structure and parentage analysis of Amazonian forest birds.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18994
Menger, J., Gerth, M., Unrein, J., Henle, K., Schlegel, M. (2017):
Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
Wilson J. Ornithol. 129 (2), 407 - 411 10.1676/16-062.1