Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1676/16-062.1 |
| Title (Primary) | Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae) |
| Author | Menger, J.; Gerth, M.; Unrein, J.; Henle, K.; Schlegel, M. |
| Source Titel | Wilson Journal of Ornithology |
| Year | 2017 |
| Department | NSF; iDiv |
| Volume | 129 |
| Issue | 2 |
| Page From | 407 |
| Page To | 411 |
| Language | englisch |
| Keywords | Amazonia, ant-following birds, cross-amplification, next-generation sequencing, nuclear markers |
| UFZ wide themes | RU1 |
| Abstract | Fourteen microsatellite loci were characterized for the Rufous-throated Antbird (Thamnophilidae: Gymnopithys rufigula), an Amazonian obligate ant-following bird. Eight novel polymorphic microsatellite loci were isolated using next-generation sequencing and six additional loci were cross-amplified using primers previously designed for other bird species. All 14 loci were screened using 40 samples from central Amazon. The number of alleles per locus ranged from 3 to 20, whilst the observed and expected heterozygosities varied from 0.200 to 0.975, and 0.186 to 0.928, respectively. No linkage disequilibrium was found among the loci. Only one locus deviated from Hardy-Weinberg equilibrium. Among the eight novel loci, six were cross-amplified in at least one additional Thamnophilidae species, whilst three loci cross-amplified in two other Dendrocolaptidae species. This set of markers will be useful for future population genetic structure and parentage analysis of Amazonian forest birds. |
| Menger, J., Gerth, M., Unrein, J., Henle, K., Schlegel, M. (2017): Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae) Wilson J. Ornithol. 129 (2), 407 - 411 10.1676/16-062.1 |
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