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Title (Primary) Detection of sulfur microparticles in bacterial cultures by flow cytometry
Author Hübschmann, T.; Vogt, C.; Till, S.; Rohwerder, T.; Sand, W.; Harms, H.; Müller, S.;
Journal Engineering in Life Sciences
Year 2007
Department ISOBIO; UMB;
Volume 7
Issue 4
Language englisch;
Keywords bacteria; flow cytometry; sulfur
Abstract Repeated flow cytometric analyses of population shifts within a bacterial consortium growing under sulfate-reducing conditions revealed unexpected and quick changes in forward scatter vs. side scatter dot plot patterns within less than 30 minutes of exposure to air. These pattern changes proceeded differently when cells were cultivated either at 30 °C or 12 °C. It was found that prominent sub-distributions, clustering profoundly in size and number of events with time, represented sulfur particles and not microbial cells. Therefore, any interpretation of flow cytometric analyses of live bacteria grown under sulfate-reducing conditions requires specific caution since sulfur particles might cause errors in data interpretation. To prevent artefacts, distinct treatments of the sample are recommended.
ID 1898
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1898
Hübschmann, T., Vogt, C., Till, S., Rohwerder, T., Sand, W., Harms, H., Müller, S. (2007):
Detection of sulfur microparticles in bacterial cultures by flow cytometry
Eng. Life Sci. 7 (4), 403 - 407