Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1093/toxsci/kfx045
Title (Primary) The transcriptome of the zebrafish embryo after chemical exposure: a meta-analysis
Author Schüttler, A.; Reiche, K.; Altenburger, R.; Busch, W. ORCID logo
Source Titel Toxicological Sciences
Year 2017
Volume 157
Issue 2
Page From 291
Page To 304
Language englisch
Keywords meta-analysis, microarray, stress response, transcriptome, toxicogenomics, zebrafish embryo
UFZ wide themes RU3;
Abstract Numerous studies have been published in the past years investigating the transcriptome of the zebrafish embryo (ZFE) upon being subjected to chemical stress. Aiming at a more mechanistic understanding of the results of such studies, knowledge about commonalities of transcript regulation in response to chemical stress is needed. Thus, our goal in this study was to identify and interpret genes and gene sets constituting a general response to chemical exposure. Therefore, we aggregated and reanalyzed published toxicogenomics data obtained with the ZFE. We found that overlap of differentially transcribed genes in response to chemical stress across independent studies is generally low and the most commonly differentially transcribed genes appear in less than 50% of all treatments across studies. However, effect size analysis revealed several genes showing a common trend of differential expression, among which genes related to calcium homeostasis emerged as key, especially in exposure settings up to 24 h post-fertilization. Additionally, we found that these and other downregulated genes are often linked to anatomical regions developing during the respective exposure period. Genes showing a trend of increased expression were, among others, linked to signaling pathways (e.g., Wnt, Fgf) as well as lysosomal structures and apoptosis. The findings of this study help to increase the understanding of chemical stress responses in the developing zebrafish embryo and provide a starting point to improve experimental designs for this model system. In future, improved time- and concentration-resolved experiments should offer better understanding of stress response patterns and access to mechanistic information.
Persistent UFZ Identifier
Schüttler, A., Reiche, K., Altenburger, R., Busch, W. (2017):
The transcriptome of the zebrafish embryo after chemical exposure: a meta-analysis
Toxicol. Sci. 157 (2), 291 - 304 10.1093/toxsci/kfx045