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Title (Primary) Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype
Author Perruchon, C.; Chatzinotas, A.; Omirou, M.; Vasileiadis, S.; Menkissoglou-Spiroudi, U.; Karpouzas, D.G.;
Journal Applied Microbiology and Biotechnology
Year 2017
Department UMB; iDiv;
Volume 101
Issue 9
Language englisch;
POF III (all) T23;
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00253-017-8128-5/MediaObjects/253_2017_8128_MOESM1_ESM.pdf
Keywords Thiabendazole; Fruit-packaging industry; Wastewaters; SIP-DGGE; Pesticide biodegradation; Sphingomonas
UFZ wide themes RU1;
Abstract Thiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial degradation of the recalcitrant and toxic to aquatics TBZ. We report the isolation, via enrichment cultures from a polluted soil, of the first bacterial consortium able to rapidly degrade TBZ and use it as a carbon source. Repeated efforts using various culture-dependent approaches failed to isolate TBZ-degrading bacteria in axenic cultures. Denaturating gradient gel electrophoresis (DGGE) and cloning showed that the consortium was composed of α-, β- and γ-Proteobacteria. Culture-independent methods including antibiotics-driven selection with DNA/RNA-DGGE, q-PCR and stable isotope probing (SIP)-DGGE identified a Sphingomonas phylotype (B13) as the key degrading member. Cross-feeding studies with structurally related chemicals showed that ring substituents of the benzimidazole moiety (thiazole or furan rings) favoured the cleavage of the imidazole moiety. LC-MS/MS analysis verified that TBZ degradation proceeds via cleavage of the imidazole moiety releasing thiazole-4-carboxamidine, which was not further transformed, and the benzoyl moiety, possibly as catechol, which was eventually consumed by the bacterial consortium as suggested by SIP-DGGE.
ID 18547
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18547
Perruchon, C., Chatzinotas, A., Omirou, M., Vasileiadis, S., Menkissoglou-Spiroudi, U., Karpouzas, D.G. (2017):
Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype
Appl. Microbiol. Biotechnol. 101 (9), 3881 - 3893