Publication Details

Reference Category Book chapters
DOI / URL link
Title (Primary) Introduction to activities and phenotypes
Title (Secondary) Hydrocarbon and lipid microbiology protocols. Activities and phenotypes
Author Musat, F.;
Publisher McGenity, T.J.; Timmis, K.N.; Nogales, B.;
Journal Springer Protocols Handbooks
Year 2017
Department ISOBIO;
Language englisch;
POF III (all) T15;
Keywords Biosurfactants; Chemotactism; Crude oil; Functional diversity; Hydrophobic phases; Lipids; Methanogenesis; Respiration rates; Sulfate reduction; Transposon mutagenesis
UFZ wide themes RU4;
UFZ inventory Leipzig, Bibliothek, Hauptlesesaal, 00525091, 18-0115 DK: 579.222.5 Hyd
Abstract Crude oil and lipids greatly influence the structure and function of microbial communities, owing to merely physical effects such as hindering the diffusion of oxygen or light to communities trapped beneath oil layers, to toxicity of the highly soluble oil hydrocarbons, or to the utilization of hydrocarbons or lipids as growth substrates by microorganisms. This chapter brings together methods to investigate the effects of crude oil or lipids on the diversity and function of microbial communities. These can be principally grouped into protocols devoted to analyze the functional diversity of microbial communities in response to hydrocarbons or lipids, protocols aiming to resolve the specific interactions of microorganisms with hydrophobic phases, protocols to study the effects of hydrocarbons or lipids on bulk metabolic activities of microbial communities, and tools to identify specific genes involved in metabolic processes. Most of the protocols presented here can be applied directly on environmental samples, or on laboratory-scale micro/mesocosms, thereby allowing a direct comparison between pristine samples and samples contaminated experimentally with hydrocarbons.
ID 18326
Persistent UFZ Identifier
Musat, F. (2017):
Introduction to activities and phenotypes
In: McGenity, T.J., Timmis, K.N., Nogales, B. (eds.)
Hydrocarbon and lipid microbiology protocols. Activities and phenotypes
Springer Protocols Handbooks
Springer, Berlin, Heidelberg, p. 1 - 6