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Title (Primary) Rectinema cohabitans gen. nov., sp. nov., a rod-shaped spirochaete isolated from an anaerobic naphthalene-degrading enrichment culture
Author Koelschbach, J.S.; Mouttaki, H.; Pickl, C.; Heipieper, H.J.; Rachel, R.; Lawson, P.A.; Meckenstock, R.U.;
Journal International Journal of Systematic and Evolutionary Microbiology
Year 2017
Department UBT;
Volume 67
Issue 5
Language englisch;
POF III (all) T41;
UFZ wide themes RU3;
Abstract The anaerobic, non-motile strain HMT was isolated from the naphthalene-degrading, sulfate-reducing enrichment culture N47. Since 20 years, strain HMT has been a stable member of culture N47 although it is neither able to degrade naphthalene nor to reduce sulfate in pure culture. The highest similarity of the 16S rRNA gene sequence of strain HMT (89%) is with a cultivated member of the family Spirochaetaceae, Treponema caldarium sp. strain H1T (DSM 7334T), an obligately anaerobic, thermophilic spirochete isolated from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA. In contrast to this strain and the majority of described spirochete species, strain HMT showed a rod-shaped morphology. Growth occurred between 12 to 50 °C (optimum 37 °C) but the isolate was not able to grow at 60 °C. The strain fermented various sugars including D-glucose, D-fructose, lactose, and sucrose. Addition of 0.1% (w/v) yeast extract or 0.1% (w/v) tryptone to the culture medium was essential for growth and could neither be replaced by the tested vitamin solutions nor by 0.1% (w/v) peptone or 0.1% (w/v) casamino acids. The DNA G+C content of the isolate was 51.5 mol%. The major fatty acids are C14:0, C18:1ω13c, C16:1ω9t, C16:1ω11c, and C16:1ω9c. Based on the unique morphology and the phylogenetic distance from the closest cultivated relative, a novel genus and species, Rectinema cohabitans gen. nov., sp. nov., is proposed. The type strain is strain HMT (=DSM 100378T =JCM 30982T).
ID 18313
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18313
Koelschbach, J.S., Mouttaki, H., Pickl, C., Heipieper, H.J., Rachel, R., Lawson, P.A., Meckenstock, R.U. (2017):
Rectinema cohabitans gen. nov., sp. nov., a rod-shaped spirochaete isolated from an anaerobic naphthalene-degrading enrichment culture
Int. J. Syst. Evol. Microbiol. 67 (5), 1288 - 1295