Publication Details
Category Text Publication
Reference Category Book chapters
DOI 10.1007/8623_2014_30
Title (Primary) Protocol for laser scanning microscopy of microorganisms on hydrocarbons
Title (Secondary) Hydrocarbon and lipid microbiology protocols. Ultrastructure and imaging
Author Neu, T.R.; Lawrence, J.R.
Publisher McGenity, T.J.; Timmis, K.N.; Nogales, B.
Journal Springer Protocols Handbooks
Year 2016
Department FLOEK
Page From 29
Page To 47
Language englisch
Keywords Bacteria; Biofilms; Colonisation of hydrocarbons; Confocal laser scanning microscopy; Deconvolution; Degradation of hydrocarbons; Digital image analysis; Fluorescence techniques; Fluorochromes; Hydrophobicity; Image analysis; Imaging; Imaging techniques; Laser scanning microscopy; Lectins; Microorganisms; Quantification; Two photon laser scanning microscopy; Visualisation
UFZ wide themes RU2;
Abstract Microbial communities in their fully hydrated state can be imaged in space and time (4-dimensionally) by laser scanning microscopy using 1-photon or 2-photon excitation. In this protocol, we provide guidance on how to examine microorganisms associated with liquid, viscous and solid hydrocarbons. Practical aspects are discussed including the material and sources, microscopy consumables, software programs and time constraints. The details of mounting samples for the upright and inverted microscope as well as options for fluorescence staining of bacteria and hydrocarbons are presented. Suggestions are made for recording images and subsequent digital image analysis. Finally, notes are added and a guideline for troubleshooting is supplied.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18196
Neu, T.R., Lawrence, J.R. (2016):
Protocol for laser scanning microscopy of microorganisms on hydrocarbons
In: McGenity, T.J., Timmis, K.N., Nogales, B. (eds.)
Hydrocarbon and lipid microbiology protocols. Ultrastructure and imaging
Springer Protocols Handbooks
Springer, Berlin, Heidelberg, p. 29 - 47