Publication Details |
| Category | Text Publication |
| Reference Category | Book chapters |
| DOI | 10.1007/8623_2014_30 |
| Title (Primary) | Protocol for laser scanning microscopy of microorganisms on hydrocarbons |
| Title (Secondary) | Hydrocarbon and lipid microbiology protocols. Ultrastructure and imaging |
| Author | Neu, T.R.; Lawrence, J.R. |
| Publisher | McGenity, T.J.; Timmis, K.N.; Nogales, B. |
| Source Titel | Springer Protocols Handbooks |
| Year | 2016 |
| Department | FLOEK |
| Page From | 29 |
| Page To | 47 |
| Language | englisch |
| Keywords | Bacteria; Biofilms; Colonisation of hydrocarbons; Confocal laser scanning microscopy; Deconvolution; Degradation of hydrocarbons; Digital image analysis; Fluorescence techniques; Fluorochromes; Hydrophobicity; Image analysis; Imaging; Imaging techniques; Laser scanning microscopy; Lectins; Microorganisms; Quantification; Two photon laser scanning microscopy; Visualisation |
| UFZ wide themes | RU2; |
| Abstract | Microbial communities in their fully hydrated state can be imaged in space and time (4-dimensionally) by laser scanning microscopy using 1-photon or 2-photon excitation. In this protocol, we provide guidance on how to examine microorganisms associated with liquid, viscous and solid hydrocarbons. Practical aspects are discussed including the material and sources, microscopy consumables, software programs and time constraints. The details of mounting samples for the upright and inverted microscope as well as options for fluorescence staining of bacteria and hydrocarbons are presented. Suggestions are made for recording images and subsequent digital image analysis. Finally, notes are added and a guideline for troubleshooting is supplied. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18196 |
| Neu, T.R., Lawrence, J.R. (2016): Protocol for laser scanning microscopy of microorganisms on hydrocarbons In: McGenity, T.J., Timmis, K.N., Nogales, B. (eds.) Hydrocarbon and lipid microbiology protocols. Ultrastructure and imaging Springer Protocols Handbooks Springer, Berlin, Heidelberg, p. 29 - 47 10.1007/8623_2014_30 |
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