Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Author Zimmermann, J.; Hübschmann, T.; Schattenberg, F.; Schumann, J.; Durek, P.; Riedel, R.; Friedrich, M.; Glauben, R.; Siegmund, B.; Radbruch, A.; Müller, S.; Chang, H.-D.;
Journal European Journal of Immunology
Year 2016
Department UMB;
Volume 46
Issue 5
Language englisch;
POF III (all) T41;
Keywords Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis
UFZ wide themes RU3;
Abstract Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.
ID 17808
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17808
Zimmermann, J., Hübschmann, T., Schattenberg, F., Schumann, J., Durek, P., Riedel, R., Friedrich, M., Glauben, R., Siegmund, B., Radbruch, A., Müller, S., Chang, H.-D. (2016):
High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Eur. J. Immunol. 46 (5), 1300 - 1303