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Title (Primary) Comparison and optimization of methods for the simultaneous extraction of DNA, RNA, proteins, and metabolites
Author Vorreiter, F.; Richter, S.; Peter, M.; Baumann, S.; von Bergen, M.; Tomm, J.M.;
Journal Analytical Biochemistry
Year 2016
Department MOLSYB;
Volume 508
Language englisch;
POF III (all) F11;
Keywords Molecular systems biology; Genomics; Transcriptomics; Proteomics; Metabolomics
UFZ wide themes RU3;
Abstract The challenge of performing a time-resolved comprehensive analysis of molecular systems has led to the quest to optimize extraction methods. When the size of a biological sample is limited, there is demand for the simultaneous extraction of molecules representing the four areas of “omics”: genomics, transcriptomics, proteomics, and metabolomics. Here we optimized a protocol for the simultaneous extraction of DNA, RNA, proteins, and metabolites and compared it with two existing protocols. Our optimization comprised the addition of a methanol/chloroform metabolite purification before the separation of DNA/RNA and proteins. Extracted DNA, RNA, proteins, and metabolites were quantitatively and/or qualitatively analyzed. Of the three methods, only the newly developed protocol yielded all biomolecule classes of adequate quantity and quality.
ID 17659
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17659
Vorreiter, F., Richter, S., Peter, M., Baumann, S., von Bergen, M., Tomm, J.M. (2016):
Comparison and optimization of methods for the simultaneous extraction of DNA, RNA, proteins, and metabolites
Anal. Biochem. 508 , 25 - 33