Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.ab.2016.05.011 |
| Title (Primary) | Comparison and optimization of methods for the simultaneous extraction of DNA, RNA, proteins, and metabolites |
| Author | Vorreiter, F.; Richter, S.; Peter, M.; Baumann, S.; von Bergen, M.; Tomm, J.M. |
| Source Titel | Analytical Biochemistry |
| Year | 2016 |
| Department | MOLSYB |
| Volume | 508 |
| Page From | 25 |
| Page To | 33 |
| Language | englisch |
| Keywords | Molecular systems biology; Genomics; Transcriptomics; Proteomics; Metabolomics |
| UFZ wide themes | RU3; |
| Abstract | The challenge of performing a time-resolved comprehensive analysis of molecular systems has led to the quest to optimize extraction methods. When the size of a biological sample is limited, there is demand for the simultaneous extraction of molecules representing the four areas of “omics”: genomics, transcriptomics, proteomics, and metabolomics. Here we optimized a protocol for the simultaneous extraction of DNA, RNA, proteins, and metabolites and compared it with two existing protocols. Our optimization comprised the addition of a methanol/chloroform metabolite purification before the separation of DNA/RNA and proteins. Extracted DNA, RNA, proteins, and metabolites were quantitatively and/or qualitatively analyzed. Of the three methods, only the newly developed protocol yielded all biomolecule classes of adequate quantity and quality. |
| Vorreiter, F., Richter, S., Peter, M., Baumann, S., von Bergen, M., Tomm, J.M. (2016): Comparison and optimization of methods for the simultaneous extraction of DNA, RNA, proteins, and metabolites Anal. Biochem. 508 , 25 - 33 10.1016/j.ab.2016.05.011 |
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