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Title (Primary) Structural and microtubule binding properties of tau mutants of frontotemporal dementias
Author Fischer, D.; Mukrasch, M.D.; von Bergen, M.; Klos-Witkowska, A.; Biernat, J.; Griesinger, C.; Mandelkow, E.; Zweckstetter, M.;
Journal Biochemistry
Year 2007
Department PROTEOM;
Volume 46
Issue 10
Language englisch;
Abstract Several mutations in the gene encoding the microtubule-associated protein tau are responsible for the formation of neurofibrillary inclusions in frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). Here we present the high-resolution characterization of the conformational properties of two FTDP-17 mutants of the four-repeat domain of tau, P301L and K280, and their properties for binding to polyanions and microtubules. Multidimensional NMR spectroscopy shows that the mutations do no lead to a significant increase in the level of -structure in their monomeric state, even though the mutations strongly promote -structure during aggregation. However, local structural changes are induced in the second repeat. These changes only weakly affect the binding to the polyanion heparin, which promotes paired helical filament formation. The extent of binding to microtubules, however, is strongly decreased. Our results demonstrate that the reversible binding of tau to microtubules involves specific interactions, which are not essential for binding to polyanions.
ID 1759
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1759
Fischer, D., Mukrasch, M.D., von Bergen, M., Klos-Witkowska, A., Biernat, J., Griesinger, C., Mandelkow, E., Zweckstetter, M. (2007):
Structural and microtubule binding properties of tau mutants of frontotemporal dementias
Biochemistry 46 (10), 2574 - 2582