Publication Details

Reference Category Journals
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Title (Primary) Isotopes in geobiochemistry: tracing metabolic pathways in microorganisms of environmental relevance with stable isotopes
Author Adrian, L.; Marco-Urrea, E.;
Journal Current Opinion in Biotechnology
Year 2016
Department ISOBIO;
Volume 41
Language englisch;
POF III (all) T41;
UFZ wide themes RU3;
Abstract Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connected to liquid chromatography (LC–MS/MS) and the explosion of microbial genome sequence information the options to apply stable isotope tracers to geobiochemical topics have multiplied. With methods at hand to analyze biochemical pathways and enzymatic functions of yet-uncultivated microorganisms even in mixed cultures, a wide field of new discoveries can be expected. Applications rely both on the high sensitivity to detect trace amounts of biological material in slow or non-growing cultures and on the high multi-dimensional resolution of LC–MS/MS to allow the separation of complex samples and to retrieve phylogenetic information. Challenges and examples of stable isotope applications to describe geobiochemical processes are reviewed. Overall, the potential is not yet sufficiently deployed.
ID 17523
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17523
Adrian, L., Marco-Urrea, E. (2016):
Isotopes in geobiochemistry: tracing metabolic pathways in microorganisms of environmental relevance with stable isotopes
Curr. Opin. Biotechnol. 41 , 19 - 25