Publication Details

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Title (Primary) Sequence Specific Primer Extension RNA Analysis (SeSPERA) for the investigation of substrate utilization of microbial communities
Author Franchini, A.G.; Nikolausz, M.; Kästner, M.;
Journal Journal of Microbiological Methods
Year 2009
Department UBT; BIOENERGIE;
Volume 79
Issue 1
Language englisch;
Keywords 16S rRNA; Community structure; Microbial activity; Multiplex detection; Radioisotope probing; Specific primer elongation
Abstract A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the RNA.
ID 175
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=175
Franchini, A.G., Nikolausz, M., Kästner, M. (2009):
Sequence Specific Primer Extension RNA Analysis (SeSPERA) for the investigation of substrate utilization of microbial communities
J. Microbiol. Methods 79 (1), 111 - 113