Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.mimet.2009.07.010 |
| Title (Primary) | Sequence Specific Primer Extension RNA Analysis (SeSPERA) for the investigation of substrate utilization of microbial communities |
| Author | Franchini, A.G.; Nikolausz, M.; Kästner, M. |
| Source Titel | Journal of Microbiological Methods |
| Year | 2009 |
| Department | UBT; BIOENERGIE |
| Volume | 79 |
| Issue | 1 |
| Page From | 111 |
| Page To | 113 |
| Language | englisch |
| Keywords | 16S rRNA; Community structure; Microbial activity; Multiplex detection; Radioisotope probing; Specific primer elongation |
| Abstract | A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the RNA. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=175 |
| Franchini, A.G., Nikolausz, M., Kästner, M. (2009): Sequence Specific Primer Extension RNA Analysis (SeSPERA) for the investigation of substrate utilization of microbial communities J. Microbiol. Methods 79 (1), 111 - 113 10.1016/j.mimet.2009.07.010 |
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