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Title (Primary) The alkaloid gramine in the anaerobic digestion process—inhibition and adaptation of the methanogenic community
Author Popp, D.; Harms, H.; Sträuber, H.;
Journal Applied Microbiology and Biotechnology
Year 2016
Department UMB;
Volume 100
Issue 16
Language englisch;
POF III (all) R31; T15;
Keywords Continuous stirred tank reactor; Biogas production; T-RFLP fingerprinting; mcrA; Reed canary grass
UFZ wide themes RU4;
Abstract As many plant secondary metabolites have antimicrobial activity, microorganisms of the anaerobic digestion process might be affected when plant material rich in these compounds is digested. Hitherto, the effects of plant secondary metabolites on the anaerobic digestion process are poorly investigated. In this study, the alkaloid gramine, a constituent of reed canary grass, was added daily to a continuous co-digestion of grass silage and cow manure. A transient decrease of the methane yield by 17 % and a subsequent recovery was observed, but no effect on other process parameters. When gramine was infrequently spiked in higher amounts, the observed inhibitory effect was even more pronounced including a 53 % decrease of the methane yield and an increase of acetic acid concentrations up to 96 mM. However, the process recovered and the process parameters were finally at initial values (methane yield around 255 LN CH4 per gram volatile solids of substrate and acetic acid concentration lower than 2 mM). The bacterial communities of the reactors remained stable upon gramine addition. In contrast, the methanogenic community changed from a well-balanced mixture of five phylotypes towards a strong dominance of Methanosarcina (more than two thirds of the methanogenic community) while Methanosaeta disappeared. Batch inhibition assays revealed that acetic acid was only converted to methane via acetoclastic methanogenesis which was more strongly affected by gramine than hydrogenotrophic methanogenesis and acetogenesis. Hence, when acetoclastic methanogenesis is the dominant pathway, a shift of the methanogenic community is necessary to digest gramine-rich plant material.
ID 17452
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17452
Popp, D., Harms, H., Sträuber, H. (2016):
The alkaloid gramine in the anaerobic digestion process—inhibition and adaptation of the methanogenic community
Appl. Microbiol. Biotechnol. 100 (16), 7311 - 7322