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Title (Primary) Hydrogen isotope fractionation as a tool to identify aerobic and anaerobic PAH biodegradation
Author Kümmel, S.; Starke, R.; Chen, G.; Musat, F.; Richnow, H.H.; Vogt, C.;
Journal Environmental Science & Technology
Year 2016
Department ISOBIO;
Volume 50
Issue 6
Language englisch;
POF III (all) T15; T41;
Supplements https://pubs.acs.org/doi/suppl/10.1021/acs.est.5b04819/suppl_file/es5b04819_si_001.pdf
UFZ wide themes RU4;
Abstract Aerobic and anaerobic polycyclic aromatic hydrocarbon (PAH) biodegradation was characterized by compound specific stable isotope analysis (CSIA) of the carbon and hydrogen isotope effects of the enzymatic reactions initiating specific degradation pathways, using naphthalene and 2-methylnaphtalene as model compounds. Aerobic activation of naphthalene and 2-methylnaphthalene by Pseudomonas putida NCIB 9816 and Pseudomonas fluorescens ATCC 17483 containing naphthalene dioxygenases was associated with moderate carbon isotope fractionation (εC = −0.8 ± 0.1‰ to −1.6 ± 0.2‰). In contrast, anaerobic activation of naphthalene by a carboxylation-like mechanism by strain NaphS6 was linked to negligible carbon isotope fractionation (εC = −0.2 ± 0.2‰ to −0.4 ± 0.3‰). Notably, anaerobic activation of naphthalene by strain NaphS6 exhibited a normal hydrogen isotope fractionation (εH = −11 ± 2‰ to −47 ± 4‰), whereas an inverse hydrogen isotope fractionation was observed for the aerobic strains (εH = +15 ± 2‰ to +71 ± 6‰). Additionally, isotope fractionation of NaphS6 was determined in an overlaying hydrophobic carrier phase, resulting in more reliable enrichment factors compared to immobilizing the PAHs on the bottle walls without carrier phase. The observed differences especially in hydrogen fractionation might be used to differentiate between aerobic and anaerobic naphthalene and 2-methylnaphthalene biodegradation pathways at PAH-contaminated field sites.
ID 17324
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17324
Kümmel, S., Starke, R., Chen, G., Musat, F., Richnow, H.H., Vogt, C. (2016):
Hydrogen isotope fractionation as a tool to identify aerobic and anaerobic PAH biodegradation
Environ. Sci. Technol. 50 (6), 3091 - 3100