Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.anaerobe.2016.03.013
Title (Primary) A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters
Author Bühligen, F.; Lucas, R.; Nikolausz, M.; Kleinsteuber, S. ORCID logo
Source Titel Anaerobe
Year 2016
Department UMB
Volume 39
Page From 114
Page To 116
Language englisch
Keywords mcrA; Community fingerprinting; BstNI; MwoI; Methanogenic archaea; Biogas
UFZ wide themes RU4;
Abstract We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17292
Bühligen, F., Lucas, R., Nikolausz, M., Kleinsteuber, S. (2016):
A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters
Anaerobe 39 , 114 - 116