Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1159/000440806 |
| Title (Primary) | Stable isotope probing approaches to study anaerobic hydrocarbon degradation and degraders |
| Author | Vogt, C.; Lueders, T.; Richnow, H.H.; Krüger, M.; von Bergen, M.; Seifert, J. |
| Source Titel | Journal of Molecular Microbiology and Biotechnology |
| Year | 2016 |
| Department | ISOBIO; MOLSYB |
| Volume | 26 |
| Issue | 1-3 |
| Page From | 195 |
| Page To | 210 |
| Language | englisch |
| Keywords | Stable isotope probing; BTEX; Syntrophic hydrocarbon degradation |
| UFZ wide themes | RU3; |
| Abstract | Stable isotope probing (SIP) techniques have become state-of-the-art in microbial ecology over the last 10 years, allowing for the targeted detection and identification of organisms, metabolic pathways and elemental fluxes active in specific processes within complex microbial communities. For studying anaerobic hydrocarbon-degrading microbial communities, four stable isotope techniques have been used so far: DNA/RNA-SIP, PLFA (phospholipid-derived fatty acids)-SIP, protein-SIP, and single-cell-SIP by nanoSIMS (nanoscale secondary ion mass spectrometry) or confocal Raman microscopy. DNA/RNA-SIP techniques are most frequently applied due to their most meaningful phylogenetic resolution. Especially using 13C-labeled benzene and toluene as model substrates, many new hydrocarbon degraders have been identified by SIP under various electron acceptor conditions. This has extended the current perspective of the true diversity of anaerobic hydrocarbon degraders relevant in the environment. Syntrophic hydrocarbon degradation was found to be a common mechanism for various electron acceptors. Fundamental concepts and recent advances in SIP are reflected here. A discussion is presented concerning how these techniques generate direct insights into intrinsic hydrocarbon degrader populations in environmental systems and how useful they are for more integrated approaches in the monitoring of contaminated sites and for bioremediation. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17257 |
| Vogt, C., Lueders, T., Richnow, H.H., Krüger, M., von Bergen, M., Seifert, J. (2016): Stable isotope probing approaches to study anaerobic hydrocarbon degradation and degraders J. Mol. Microbiol. Biotechnol. 26 (1-3), 195 - 210 10.1159/000440806 |
|