Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1080/15476286.2015.1132139
Title (Primary) U6 snRNA intron insertion occurred multiple times during fungi evolution
Author Canzler, S.; Stadler, P.F.; Hertel, J.
Source Titel RNA Biology
Year 2016
Department MOLSYB
Volume 13
Issue 2
Page From 119
Page To 127
Language englisch
Keywords Fungi; homology search; intron; snRNA; snRNA evolution
UFZ wide themes RU3;
Abstract U6 small nuclear RNAs are part of the splicing machinery. They exhibit several unique features setting them appart from other snRNAs. Reports of introns in structured non-coding RNAs have been very rare. U6 genes, however, were found to be interrupted by an intron in several Schizosaccharomyces species and in 2 Basidiomycota. We conducted a homology search across 147 currently available fungal genome and identified the U6 genes in all but 2 of them. A detailed comparison of their sequences and predicted secondary structures showed that intron insertion events in the U6 snRNA were much more common in the fungal lineage than previously thought. Their positional distribution across the entire mature snRNA strongly suggests a large number of independent events. All the intron sequences reported here show canonical splice site and branch site motifs indicating that they require the splicesomal pathway for their removal.
Persistent UFZ Identifier
Canzler, S., Stadler, P.F., Hertel, J. (2016):
U6 snRNA intron insertion occurred multiple times during fungi evolution
RNA Biol. 13 (2), 119 - 127 10.1080/15476286.2015.1132139