Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) PIPINO: a software package to facilitate the identification of protein-protein interactions from affinity purification mass spectrometry data
Author Kalkhof, S.; Schildbach, S.; Blumert, C.; Horn, F.; von Bergen, M.; Labudde, D.;
Journal BioMed Research International
Year 2016
Department MOLSYB;
Volume 2016
Language englisch;
POF III (all) F11;
UFZ wide themes RU3;
Abstract The functionality of most proteins is regulated by protein-protein interactions. Hence, the comprehensive characterization of the interactome is the next milestone on the path to understand the biochemistry of the cell. A powerful method to detect protein-protein interactions is a combination of coimmunoprecipitation or affinity purification with quantitative mass spectrometry. Nevertheless, both methods tend to precipitate a high number of background proteins due to nonspecific interactions. To address this challenge the software Protein-Protein-Interaction-Optimizer (PIPINO) was developed to perform an automated data analysis, to facilitate the selection of bona fide binding partners, and to compare the dynamic of interaction networks. In this study we investigated the STAT1 interaction network and its activation dependent dynamics. Stable isotope labeling by amino acids in cell culture (SILAC) was applied to analyze the STAT1 interactome after streptavidin pull-down of biotagged STAT1 from human embryonic kidney 293T cells with and without activation. Starting from more than 2,000 captured proteins 30 potential STAT1 interaction partners were extracted. Interestingly, more than 50% of these were already reported or predicted to bind STAT1. Furthermore, 16 proteins were found to affect the binding behavior depending on STAT1 phosphorylation such as STAT3 or the importin subunits alpha 1 and alpha 6.
ID 17201
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17201
Kalkhof, S., Schildbach, S., Blumert, C., Horn, F., von Bergen, M., Labudde, D. (2016):
PIPINO: a software package to facilitate the identification of protein-protein interactions from affinity purification mass spectrometry data
Biomed Res. Int. 2016 , art. 2891918