Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.cca.2015.12.005
Title (Primary) Proteome profiles of HDL particles of patients with chronic heart failure are associated with immune response and also include bacteria proteins
Author Oberbach, A.; Adams, V.; Schlichting, N.; Heinrich, M.; Kullnick, Y.; Lehmann, S.; Feder, S.; Correia, J.C.; Mohr, F.-W.; Völker, U.; Jehmlich, N. ORCID logo
Source Titel Clinica Chimica Acta
Year 2016
Department MOLSYB
Volume 453
Page From 114
Page To 122
Language englisch
Keywords High density lipoprotein (HDL); (Meta)proteomics; HDL variability; HDL functionality
UFZ wide themes RU3;
Abstract

Besides modulation of reverse cholesterol transport, high density lipoprotein (HDL) is able to modulate vascular function by stimulating endothelial nitric oxide synthase. Recently, it could be documented that this function of HDL was significantly impaired in patients with chronic heart failure (CHF). We investigated alterations in the HDL proteome in CHF patients. Therefore, HDL was isolated from 5 controls (HDLhealthy) and 5 CHF patients of NYHA-class IIIb (HDLCHF). Proteome analysis of HDL particles was performed by two-dimensional liquid chromatography–mass spectrometry (SCX/RP LC–MS/MS). In total, we identified 494 distinct proteins, of which 107 proteins were commonly found in both groups (HDLCHF and HDLhealthy) indicating a high inter-subject variability across HDL particles. Several important proteins (e.g. ITGA2, APBA1 or A2M) varied in level. Functional analysis revealed regulated pathways. A minor proportion of bacteria-derived proteins were also identified in the HDL-particles.

The extension of the list of HDL-associated proteins allows besides their mere description new insights into alterations in HDL function in diseases. In addition, the detection of bacterial proteins bound to HDL will broaden our view of HDL not only as a cholesterol carrier but also as a carrier of proteins.

Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17147
Oberbach, A., Adams, V., Schlichting, N., Heinrich, M., Kullnick, Y., Lehmann, S., Feder, S., Correia, J.C., Mohr, F.-W., Völker, U., Jehmlich, N. (2016):
Proteome profiles of HDL particles of patients with chronic heart failure are associated with immune response and also include bacteria proteins
Clin. Chim. Acta 453 , 114 - 122 10.1016/j.cca.2015.12.005