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Title (Primary) Characterization of phenol and cresol biodegradation by compound-specific stable isotope analysis
Author Wei, X.; Gilevska, T.; Wetzig, F.; Dorer, C.; Richnow, H.-H.; Vogt, C.;
Journal Environmental Pollution
Year 2016
Department ISOBIO;
Volume 210
Language englisch;
POF III (all) T41;
Keywords LC-IRMS; Carbon isotope fractionation; Benzylsuccinate synthase; Monooxygenase; p-Cresol methyl hydroxylase
UFZ wide themes RU3;
Abstract Microbial degradation of phenol and cresols can occur under oxic and anoxic conditions by different degradation pathways. One recent technique to take insight into reaction mechanisms is compound-specific isotope analysis (CSIA). While enzymes and reaction mechanisms of several degradation pathways have been characterized in (bio)chemical studies, associated isotope fractionation patterns have been rarely reported, possibly due to constraints in current analytical methods. In this study, carbon enrichment factors and apparent kinetic isotope effects (AKIEc) of the initial steps of different aerobic and anaerobic phenol and cresols degradation pathways were analyzed by isotope ratio mass spectrometry connected with liquid chromatography (LC-IRMS). Significant isotope fractionation was detected for aerobic ring hydroxylation, anoxic side chain hydroxylation, and anoxic fumarate addition, while anoxic carboxylation reactions produced small and inconsistent fractionation. The results suggest that several microbial degradation pathways of phenol and cresols are detectable in the environment by CSIA
ID 17016
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17016
Wei, X., Gilevska, T., Wetzig, F., Dorer, C., Richnow, H.-H., Vogt, C. (2016):
Characterization of phenol and cresol biodegradation by compound-specific stable isotope analysis
Environ. Pollut. 210 , 166 - 173