Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy
Author Schkolnik, G.; Schmidt, M.; Mazza, M.G.; Harnisch, F.; Musat, N.;
Journal PLOS ONE
Year 2015
Department ISOBIO; UMB;
Volume 10
Issue 12
Language englisch;
POF III (all) T41; T15;
Data links https://doi.org/10.5061/dryad.8sc52
UFZ wide themes ProVIS; RU3;
Abstract Shewanella oneidensis MR-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. When allowed to anaerobically colonize an Ag/AgCl solid interface, S. oneidensis has precipitated silver nanoparticles (AgNp), thus providing the means for a surface enhanced confocal Raman microscopy (SECRaM) investigation of its biofilm. The result is the in-situ chemical mapping of the biofilm as it developed over time, where the distribution of cytochromes, reduced and oxidized flavins, polysaccharides and phosphate in the undisturbed biofilm is monitored. Utilizing AgNp bio-produced by the bacteria colonizing the Ag/AgCl interface, we could perform SECRaM while avoiding the use of a patterned or roughened support or the introduction of noble metal salts and reducing agents. This new method will allow a spatially and temporally resolved chemical investigation not only of Shewanella biofilms at an insoluble electron acceptor, but also of other noble metal nanoparticle-precipitating bacteria in laboratory cultures or in complex microbial communities in their natural habitats.
ID 16997
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16997
Schkolnik, G., Schmidt, M., Mazza, M.G., Harnisch, F., Musat, N. (2015):
In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy
PLOS One 10 (12), e0145871