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Title (Primary) Changing feeding regimes to demonstrate flexible biogas production: effects on process performance, microbial community structure, and methanogenesis pathways
Author Mulat, D.G.; Jacobi, H.F.; Feilberg, A.; Adamsen, A.P.S.; Richnow, H.-H.; Nikolausz, M.;
Journal Applied and Environmental Microbiology
Year 2016
Department ISOBIO; UMB;
Volume 82
Issue 2
Language englisch;
POF III (all) R31; T15;
UFZ wide themes RU4;
Abstract Flexible biogas production that adapts biogas output to energy demand can be regulated by changing feeding regimes. In this study, the effect of change in feeding intervals on process performance, microbial community structure and methanogenesis pathway was investigated. Three different feeding regimes (once daily, every second day and every 2 hours) at the same organic loading rate were studied in continuous stirred tank reactors treating distillers dried grains with solubles. Higher amount of biogas was produced after feeding in the less frequently fed reactors (once per day and every second day) whereas it remained constant in the more frequently fed reactor (every 2 hours), indicating the suitability of the former for production of biogas flexibly. Compared to the conventional more frequent feeding regimes, higher methane yield of up to 14% and improved process stability against organic overloading were achieved by employing less frequent feeding regimes. Bacteria and methanogenic archaea community structures were monitored by terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA and mcrA genes, respectively. The results showed that the composition of the bacterial community varied under the different feeding regimes and the observed T-RFLP patterns were best explained by the difference in total ammonia nitrogen concentration, H2 level and pH value. However, the methanogenic community remained stable under all feeding regimes, with the dominance of Methanosarcina followed by Methanobacterium genus. Stable isotope analysis showed that the average methane produced during each feeding event by acetoclastic and hydrogenotrophic methanogenesis was not influenced under the three feeding regimes.
ID 16799
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16799
Mulat, D.G., Jacobi, H.F., Feilberg, A., Adamsen, A.P.S., Richnow, H.-H., Nikolausz, M. (2016):
Changing feeding regimes to demonstrate flexible biogas production: effects on process performance, microbial community structure, and methanogenesis pathways
Appl. Environ. Microb. 82 (2), 438 - 449