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Title (Primary) Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation
Author Pagani, M.; Rockstroh, M.; Schuster, M.; Rossetti, G.; Moro, M.; Crosti, M.; Tomm, J.M.;
Journal Proteomics
Year 2015
Department PROTEOM;
Volume 15
Issue 21
Language englisch;
POF III (all) F11;
Keywords Cell biology; Human T-lymphocytes; Immunology; LC-MS/MS; Proteome reference map; Th1-cells
UFZ wide themes RU3;
Abstract The differentiation of human CD4+ T cells into T helper cell subtypes and regulatory T cells is crucial to the immune response. Among subtypes, Th1 cells are dominant, representing approximately 50% of all lymphocytes. Thus far, most global proteomic studies have used only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or used gel-based approaches. These studies have shed light on molecular details of certain aspects of the proteome; nevertheless a global analysis of high purity primary naïve and Th1 cells by LC-MS/MS is required to provide a reference dataset for proteome-based T cell subtype characterization. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatic comparison to naïve cells reveals changes in cell metabolism and the ubiquitination pathway upon T cell differentiation. All MS data have been deposited in the ProteomeXchange with identifier PXD001066 (http://proteomecentral.proteomexchange.org/dataset/PXD001066).
ID 16789
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16789
Pagani, M., Rockstroh, M., Schuster, M., Rossetti, G., Moro, M., Crosti, M., Tomm, J.M. (2015):
Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation
Proteomics 15 (21), 3644 - 3647