Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00253-015-6996-0 |
| Document | Shareable Link |
| Title (Primary) | Metabolic and microbial community dynamics during the anaerobic digestion of maize silage in a two-phase process |
| Author | Sträuber, H.; Lucas, R.; Kleinsteuber, S.
|
| Source Titel | Applied Microbiology and Biotechnology |
| Year | 2016 |
| Department | UMB |
| Volume | 100 |
| Issue | 1 |
| Page From | 479 |
| Page To | 491 |
| Language | englisch |
| Keywords | Acidogenesis; 454 Pyrosequencing; T-RFLP; Anaerobic fermentation; Lactic acid; Volatile fatty acids |
| UFZ wide themes | RU4; |
| Abstract | Two-phasic anaerobic digestion processes (hydrolysis/acidogenesis separated from acetogenesis/methanogenesis) can be used for biogas production on demand or a combined chemicals/bioenergy production. For an effective process control, detailed knowledge about the microbial catalysts and their correlation to process conditions is crucial. In this study, maize silage was digested in a two-phase process and interrelationships between process parameters and microbial communities were revealed. In the first-phase reactor, alternating metabolic periods were observed which emerged independently from the feeding frequency. During the L-period, up to 11.8 g L-1 lactic acid was produced which significantly correlated to lactic acid bacteria of the genus Lactobacillus as the most abundant community members. During the alternating G-period, the production of volatile fatty acids (up to 5.3, 4.0 and 3.1 g L−1 for propionic, n-butyric and n-caproic acid, respectively) dominated accompanied by a high gas production containing up to 28 % hydrogen. The relative abundance of various Clostridiales increased during this metabolic period. In the second-phase reactor, the metabolic fluctuations of the first phase were smoothed out resulting in a stable biogas production as well as stable bacterial and methanogenic communities. However, the biogas composition followed the metabolic dynamics of the first phase: the hydrogen content increased during the L-period whereas highest CH4/CO2 ratios (up to 2.8) were reached during the G-period. Aceticlastic Methanosaeta as well as hydrogenotrophic Methanoculleus and Methanobacteriaceae were identified as dominant methanogens. Consequently, a directed control of the first-phase stabilizing desired metabolic states can lead to an enhanced productivity regarding chemicals and bioenergy. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16771 |
| Sträuber, H., Lucas, R., Kleinsteuber, S. (2016): Metabolic and microbial community dynamics during the anaerobic digestion of maize silage in a two-phase process Appl. Microbiol. Biotechnol. 100 (1), 479 - 491 10.1007/s00253-015-6996-0 |
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