Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/pmic.201400614
Title (Primary) Metaproteomics and metabolomics analyses of chronically petroleum-polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
Author Bargiela, R.; Herbst, F.-A.; Martínez-Martínez, M.; Seifert, J.; Rojo, D.; Cappello, S.; Genovese, M.; Crisafi, F.; Denaro, R.; Chernikova, T.N.; Barbas, C.; von Bergen, M.; Yakimov, M.M.; Ferrer, M.; Golyshin, P.N.
Source Titel Proteomics
Year 2015
Department PROTEOM
Volume 15
Issue 20
Page From 3508
Page To 3520
Language englisch
Keywords Anaerobic; Crude oil; Hydrocarbonoclastic; Mediterranean Sea; Metabolomics; Microbiology
UFZ wide themes RU3;
Abstract Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi-enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial populations towards the large-scale chronic pollution is yet to be defined, particularly in anaerobic and micro-aerophilic sites. Here, we provide an insight into the microbial metabolism in sediments from three chronically polluted marine sites along the coastline of Italy: the Priolo oil terminal/refinery site (near Siracuse, Sicily), harbour of Messina (Sicily) and shipwreck of MT Haven (near Genoa). Using shotgun metaproteomics and community metabolomics approaches, the presence of 651 microbial proteins and 4776 metabolite mass features have been detected in these three environments, revealing a high metabolic heterogeneity between the investigated sites. The proteomes displayed the prevalence of anaerobic metabolisms that were not directly related with petroleum biodegradation, indicating that in the absence of oxygen, biodegradation is significantly suppressed. This suppression was also suggested by examining the metabolome patterns. The proteome analysis further highlighted the metabolic coupling between methylotrophs and sulphate reducers in oxygen-depleted petroleum-polluted sediments.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16759
Bargiela, R., Herbst, F.-A., Martínez-Martínez, M., Seifert, J., Rojo, D., Cappello, S., Genovese, M., Crisafi, F., Denaro, R., Chernikova, T.N., Barbas, C., von Bergen, M., Yakimov, M.M., Ferrer, M., Golyshin, P.N. (2015):
Metaproteomics and metabolomics analyses of chronically petroleum-polluted sites reveal the importance of general anaerobic processes uncoupled with degradation
Proteomics 15 (20), 3508 - 3520 10.1002/pmic.201400614