Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1021/acs.est.5b02222 |
Title (Primary) | Transformation pathways of the recalcitrant pharmaceutical compound carbamazepine by the white-rot fungus Pleurotus ostreatus: Effects of growth conditions |
Author | Golan-Rozen, N.; Seiwert, B.; Riemenschneider, C.; Reemtsma, T.; Chefetz, B.; Hadar, Y. |
Journal | Environmental Science & Technology |
Year | 2015 |
Department | ANA |
Volume | 49 |
Issue | 20 |
Page From | 12351 |
Page To | 12362 |
Language | englisch |
Supplements | https://pubs.acs.org/doi/suppl/10.1021/acs.est.5b02222/suppl_file/es5b02222_si_001.pdf |
UFZ wide themes | RU3; |
Abstract | The widely used anticonvulsant pharmaceutical carbamazepine is recalcitrant in many environmental niches and thus poses a challenge in wastewater treatment. We followed the decomposition of carbamazepine by the white-rot fungus Pleurotus ostreatus in liquid culture compared to solid-state fermentation on lignocellulosic substrate where different enzymatic systems are active. Carbamazepine metabolites were identified using liquid chromatography–high-resolution mass spectrometry (LC-Q-TOF-MS). In liquid culture, carbamazepine was only transformed to 10,11-epoxy carbamazepine and 10,11-dihydroxy carbamazepine as a dead-end product. During solid-state fermentation, carbamazepine metabolism resulted in the generation of an additional 22 transformation products, some of which are toxic. Under solid-state-fermentation conditions, 10,11-epoxy carbamazepine was further metabolized via acridine and 10,11-dihydroxy carbamazepine pathways. The latter was further metabolized via five subpathways. When 14C-carbonyl-labeled carbamazepine was used as the substrate, 14C-CO2 release amounted to 17.4% of the initial radioactivity after 63 days of incubation. The proposed pathways were validated using metabolites (10,11-epoxy carbamazepine, 10,11-dihydroxy carbamazepine, and acridine) as primary substrates and following their fate at different time points. This work highlights the effect of growth conditions on the transformation pathways of xenobiotics. A better understanding of the fate of pollutants during bioremediation treatments is important for establishment of such technologies. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16755 |
Golan-Rozen, N., Seiwert, B., Riemenschneider, C., Reemtsma, T., Chefetz, B., Hadar, Y. (2015): Transformation pathways of the recalcitrant pharmaceutical compound carbamazepine by the white-rot fungus Pleurotus ostreatus: Effects of growth conditions Environ. Sci. Technol. 49 (20), 12351 - 12362 |