Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1002/cyto.a.22656 |
Title (Primary) | Facilitated gate setting by sequential dot plot scanning |
Author | Günther, S.; Müller, S. |
Source Titel | Cytometry Part A |
Year | 2015 |
Department | UMB |
Volume | 87 |
Issue | 7 |
Page From | 661 |
Page To | 664 |
Language | englisch |
Supplements | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0001-suppinfo1.doc https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0002-suppinfo2.doc https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0003-suppinfo3.tiff https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0004-suppinfo4.tiff https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0005-suppinfo5.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0006-suppinfo6.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcyto.a.22656&file=cytoa22656-sup-0007-suppinfo7.docx |
Keywords | Key terms: data analysis; gate setting; 2D-dot plot evaluation; microbial communities |
UFZ wide themes | RU4; |
Abstract | Microbial communities comprising thousands of unknown organisms can be studied flow cytometrically by applying just one fluorescent parameter and using scatter characteristics of cells. Resulting 2D-plots need to represent high numbers of cells to detect the many subcommunities, even rare ones that might be present in the sample. Evaluation of such data can be faulty and subjective due to the low number of parameters available for data discrimination and the high numbers of overlaying events. Here, we describe a procedure that helps to evaluate such data using facilitated gate setting by sequential dot-plot scanning. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16355 |
Günther, S., Müller, S. (2015): Facilitated gate setting by sequential dot plot scanning Cytom. Part A 87 (7), 661 - 664 10.1002/cyto.a.22656 |