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Title (Primary) Facilitated gate setting by sequential dot plot scanning
Author Günther, S.; Müller, S.;
Journal Cytometry Part A
Year 2015
Department UMB;
Volume 87
Issue 7
Language englisch;
POF III (all) R31;
Keywords Key terms: data analysis; gate setting; 2D-dot plot evaluation; microbial communities
UFZ wide themes RU4;
Abstract Microbial communities comprising thousands of unknown organisms can be studied flow cytometrically by applying just one fluorescent parameter and using scatter characteristics of cells. Resulting 2D-plots need to represent high numbers of cells to detect the many subcommunities, even rare ones that might be present in the sample. Evaluation of such data can be faulty and subjective due to the low number of parameters available for data discrimination and the high numbers of overlaying events. Here, we describe a procedure that helps to evaluate such data using facilitated gate setting by sequential dot-plot scanning.
ID 16355
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16355
Günther, S., Müller, S. (2015):
Facilitated gate setting by sequential dot plot scanning
Cytom. Part A 87 (7), 661 - 664