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Title (Primary) Potential of wood-rotting fungi to attack polystyrene sulfonate and its depolymerisation by Gloeophyllum trabeum via hydroquinone-driven Fenton chemistry
Author Krueger, M.C.; Hofmann, U.; Moeder, M.; Schlosser, D.;
Journal PLOS ONE
Year 2015
Department UMB; ANA;
Volume 10
Issue 7
Language englisch;
POF III (all) T41;
Supplements https://ndownloader.figshare.com/articles/1473926/versions/1
UFZ wide themes RU3;
Abstract Synthetic polymers often pose environmental hazards due to low biodegradation rates and resulting accumulation. In this study, a selection of wood-rotting fungi representing different lignocellulose decay types was screened for oxidative biodegradation of the polymer polystyrene sulfonate (PSS). Brown-rot basidiomycetes showed PSS depolymerisation of up to 50 % reduction in number-average molecular mass (Mn) within 20 days. In-depth investigations with the most efficient depolymeriser, a Gloeophyllum trabeum strain, pointed at extracellular hydroquinone-driven Fenton chemistry responsible for depolymerisation. Detection of hydroxyl radicals present in the culture supernatants showed good compliance with depolymerisation over the time course of PSS degradation. 2,5-Dimethoxy-1,4-hydroquinone (2,5-DMHQ), which was detected in supernatants of active cultures via liquid chromatography and mass spectrometry, was demonstrated to drive the Fenton processes in G. trabeum cultures. Up to 80% reduction in Mn of PSS where observed when fungal cultures were additionally supplemented with 2,5-dimethoxy benzoquinone, the oxidized from of 2,5-DMHQ. Furthermore, 2,5-DMHQ could initiate the Fenton's reagent-mediated PSS depolymerisation in cell-free systems. In contrast, white-rot fungi were unable to cause substantial depolymerising effects despite the expression of lignin-modifying exo-enzymes. Detailed investigations with laccase from Trametes versicolor revealed that only in presence of certain redox mediators limited PSS depolymerisation occurred. Our results indicate that brown-rot fungi might be suitable organisms for the biodegradation of recalcitrant synthetic polymeric pollutants.
ID 16333
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16333
Krueger, M.C., Hofmann, U., Moeder, M., Schlosser, D. (2015):
Potential of wood-rotting fungi to attack polystyrene sulfonate and its depolymerisation by Gloeophyllum trabeum via hydroquinone-driven Fenton chemistry
PLOS One 10 (7), e0131773