Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1021/es5056484
Title (Primary) Impact of dissolved organic matter on bacterial tactic motility, attachment, and transport
Author Jimenez-Sanchez, C.; Wick, L.Y.; Cantos, M.; Ortega-Calvo, J.-J.
Source Titel Environmental Science & Technology
Year 2015
Department UMB
Volume 49
Issue 7
Page From 4498
Page To 4505
Language englisch
UFZ wide themes RU3;
Abstract Bacterial dispersal is a key driver of the ecology of microbial contaminant degradation in soils. This work investigated the role of dissolved organic matter (DOM) in the motility, attachment, and transport of the soil bacterium Pseudomonas putida G7 in saturated porous media. The study is based on the hypothesis that DOM quality is critical to triggering tactic motility and, consequently, affects bacterial transport and dispersal. Sunflower root exudates, humic acids (HA), and the synthetic oleophilic fertilizer S-200 were used as representatives of fresh, weathered, and artificially processed DOM with high nitrogen and phosphorus contents, respectively. We studied DOM levels of 16–130 mg L–1, which are representative of DOM concentrations typically found in agricultural soil pore water. In contrast to its responses to HA and S-200, strain G7 exhibited a tactic behavior toward root exudates, as quantified by chemotaxis assays and single-cell motility observations. All DOM types promoted bacterial transport through sand at high concentrations (∼130 mg L–1). At low DOM concentrations (∼16 mg L–1), the enhancement occurred only in the presence of sunflower root exudates, and this enhancement did not occur with G7 bacteria devoid of flagella. Our results suggest that tactic DOM effectors strongly influence bacterial transport and the interception probability of motile bacteria by collector surfaces.
Persistent UFZ Identifier
Jimenez-Sanchez, C., Wick, L.Y., Cantos, M., Ortega-Calvo, J.-J. (2015):
Impact of dissolved organic matter on bacterial tactic motility, attachment, and transport
Environ. Sci. Technol. 49 (7), 4498 - 4505 10.1021/es5056484