Publication Details

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Title (Primary) MetaProSIP: Automated inference of stable isotope incorporation rates in proteins for functional metaproteomics
Author Sachsenberg, T.; Herbst, F.-A.; Taubert, M.; Kermer, R.; Jehmlich, N.; von Bergen, M.; Seifert, J.; Kohlbacher, O.;
Journal Journal of Proteome Research
Year 2015
Department PROTEOM;
Volume 14
Issue 2
Language englisch;
POF III (all) T41;
Keywords mass spectrometry; bioinformatics; protein-based stable isotope probing; functional metaproteomics; microbial ecology; elemental flux
UFZ wide themes RU3;
Abstract We propose a joint experimental and theoretical approach to the automated reconstruction of elemental fluxes in microbial communities. While stable isotope probing of proteins (protein-SIP) has been successfully applied to study interactions and elemental carbon and nitrogen fluxes, the volume and complexity of mass spectrometric data in protein-SIP experiments pose new challenges for data analysis. Together with a flexible experimental setup, the novel bioinformatics tool MetaProSIP offers an automated high-throughput solution for a wide range of 13C or 15N protein-SIP experiments with special emphasis on the analysis of metaproteomic experiments where differential labeling of organisms can occur. The information calculated in MetaProSIP includes the determination of multiple relative isotopic abundances, the labeling ratio between old and new synthesized proteins, and the shape of the isotopic distribution. These parameters define the metabolic capacities and dynamics within the investigated microbial culture. MetaProSIP features a high degree of reproducibility, reliability, and quality control reporting. The ability to embed into the OpenMS framework allows for flexible construction of custom-tailored workflows. Software and documentation are available under an open-source license at www.openms.de/MetaProSIP.
ID 15927
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15927
Sachsenberg, T., Herbst, F.-A., Taubert, M., Kermer, R., Jehmlich, N., von Bergen, M., Seifert, J., Kohlbacher, O. (2015):
MetaProSIP: Automated inference of stable isotope incorporation rates in proteins for functional metaproteomics
J. Proteome Res. 14 (2), 619 - 627