Publication Details |
| Category | Text Publication |
| Reference Category | Book chapters |
| DOI | 10.1007/978-1-4939-0467-9_4 |
| Title (Primary) | Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy |
| Title (Secondary) | Microbial biofilms: methods and protocols |
| Author | Neu, T.R.; Lawrence, J.R. |
| Publisher | Donelli, G. |
| Journal | Methods in Molecular Biology |
| Year | 2014 |
| Department | FLOEK |
| Volume | 1147 |
| Page From | 43 |
| Page To | 64 |
| Language | englisch |
| Keywords | Biofilm; Bioaggregate; Biofilm matrix; Extracellular polymeric substances (EPS); Confocal laser scanning microscopy (CLSM); Laser scanning microscopy (LSM); Fluorescence; Fluorescence lectin-binding analysis (FLBA) |
| UFZ wide themes | RU2; |
| Abstract | The extracellular constituents in bioaggregates and biofi lms can be imaged four dimensionally by using laser scanning microscopy. In this protocol we provide guidance on how to examine the various extracellular compartments in between microbial cells and communities associated with interfaces. The current options for fl uorescence staining of matrix compounds and extracellular microhabitats are presented. Furthermore, practical aspects are discussed and useful notes are added. The chapter ends with a brief introduction to other approaches for EPS analysis and an outlook for future needs. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15572 |
| Neu, T.R., Lawrence, J.R. (2014): Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy In: Donelli, G. (ed.) Microbial biofilms: methods and protocols Methods in Molecular Biology 1147 Springer Nature, p. 43 - 64 |
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