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Title (Primary) An efficient thermotolerant and halophilic biosurfactant-producing bacterium isolated from Dagang oil field for MEOR application
Author Wu, L.; Yao, J.; Jain, R.K.; Duan, X.; Richnow, H.H.;
Journal International Journal of Current Microbiology and Applied Sciences
Year 2014
Department ISOBIO;
Volume 3
Issue 7
Language englisch;
POF III (all) R31; T15;
Keywords Biosurfactant; Bacillus subtilis; stability; core flood study; oil degradation; MEOR
UFZ wide themes RU4;
Abstract An efficient biosurfactant-producing, thermotolerant and halophillic bacterium was isolated and cultured from petroleum reservoir of Dagang oil field, using haemolytic assay, and the qualitative oil-displacement test. On the basis of 16S rDNA analysis, the isolate was identified as Bacillus subtilis BS2. This bacterium was able to produce a type of biosurfactant which could reduce the surface tension of the culture broth from 70.87 to 28.97 mN m-1 after 8 d of incubation at 37 ºC and to 36.15 mN m-1 after 20 d of incubation at 55 ºC, respectively. The biosurfactant offering potential for biotechnology as it showed stability at high temperature, a wide range of pH and salt concentrations. The FT-IR spectrum of extracted biosurfactant tentatively characterized the biosurfactant as glycolipid derivative. Elemental analysis of the biosurfactant by EDS revealed that the biosurfactant was anionic in nature. Biodegradation experiments with crude oil suggested a preferential utilisation of n-alkanes as a carbon source by BS2 strain upon the synthesis of biosurfactant. Core flood studies for oil release indicated 9.6% of additional oil recovery during water flooding at 37 ºC and 7.2% of additional oil recovery at 55 ºC. The isolated Bacillus subtilis BS2 strain has the potential for application for MEOR through water flooding in China s oil fields.
ID 15513
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15513
Wu, L., Yao, J., Jain, R.K., Duan, X., Richnow, H.H. (2014):
An efficient thermotolerant and halophilic biosurfactant-producing bacterium isolated from Dagang oil field for MEOR application
International Journal of Current Microbiology and Applied Sciences 3 (7), 586 - 599