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Title (Primary) Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays
Author Pannier, A.; Soltmann, U.; Soltmann, B.; Altenburger, R.; Schmitt-Jansen, M.;
Journal Journal of Materials Chemistry B
Year 2014
Department BIOTOX;
Volume 45
Issue 2
Language englisch;
POF III (all) T32;
UFZ wide themes RU2;
Abstract Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca2+-ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a non-contact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the potential of the hybrid matrix for the design of cell-based detection systems. The herbicide atrazine as well as copper ions have been used as model toxicants. Short-term toxicity tests (exposure time: 1 h) have been carried out using atrazine and changes in chlorophyll a (Chl a) fluorescence were measured by imaging pulse amplitude modulated-fluorometry (Imaging-PAM). C. vulgaris cells immobilized within alginate/silica hydrogels demonstrated a highly reproducible response pattern and compared well to freely suspended cells. Activity and response sensitivity of immobilized cells to atrazine was largely maintained for up to 8 weeks, especially when stored under cool conditions in the dark. Furthermore, immobilized cells could be repeatingly used for short-term toxicity tests as atrazine produces a reversible inhibition of photosynthesis.
ID 15461
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15461
Pannier, A., Soltmann, U., Soltmann, B., Altenburger, R., Schmitt-Jansen, M. (2014):
Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays
J. Mat. Chem. B 45 (2), 7896 - 7909